{"id":8108,"date":"2024-06-22T19:01:01","date_gmt":"2024-06-22T11:01:01","guid":{"rendered":""},"modified":"2024-06-22T19:01:01","modified_gmt":"2024-06-22T11:01:01","slug":"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790","status":"publish","type":"post","link":"https:\/\/mushiming.com\/8108.html","title":{"rendered":"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790"},"content":{"rendered":"
\n

\"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790<\/p>\n

\u519c\u4e1a\u571f\u58e4\u5fae\u751f\u7269\u6838\u5fc3\u83cc\u7fa4\u80fd\u591f\u63d0\u5347\u571f\u58e4\u751f\u6001\u529f\u80fd<\/h2>\n

Abundance of kinless hubs within soil microbial networks are associated with high functional potential in agricultural ecosystems<\/strong><\/p>\n

Environment International<\/strong><\/p>\n

Impact Factor 7.943<\/strong><\/p>\n

DOI\uff1ahttps:\/\/doi.org\/10.1016\/j.envint.2020.<\/p>\n

\u53d1\u8868\u65e5\u671f\uff1a2020-06-25<\/p>\n

\u7b2c\u4e00\u4f5c\u8005\uff1aYu Shi (\u65f6\u7389)a<\/sup><\/p>\n

\u901a\u8baf\u4f5c\u8005\uff1aHaiyan Chu (\u891a\u6d77\u71d5) (hychu@issas.ac.cn)a,h, <\/em><\/sup>,Yong-Guan Zhu(\u6731\u6c38\u5b98)(ygzhu@iue.ac.cn)d,e,<\/sup><\/em><\/p>\n

\u5408\u4f5c\u4f5c\u8005: Manuel Delgado-Baquerizo, Yuntao Li(\u674e\u4e91\u6d9b), Yunfeng Yang, Josep Pe\u00f1uelas<\/p>\n

\u4e3b\u8981\u5355\u4f4d\uff1a<\/p>\n

a<\/sup> \u4e2d\u56fd\u79d1\u5b66\u9662\u5357\u4eac\u571f\u58e4\u7814\u7a76\u6240\u571f\u58e4\u4e0e\u519c\u4e1a\u53ef\u6301\u7eed\u53d1\u5c55\u56fd\u5bb6\u91cd\u70b9\u5b9e\u9a8c\u5ba4(State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 71 East Beijing Road, Nanjing , China)<\/p>\n

d<\/sup> \u4e2d\u56fd\u79d1\u5b66\u9662\u57ce\u5e02\u73af\u5883\u7814\u7a76\u6240\u57ce\u5e02\u73af\u5883\u4e0e\u5065\u5eb7\u91cd\u70b9\u5b9e\u9a8c\u5ba4(Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen , China)<\/p>\n

e<\/sup> \u4e2d\u56fd\u79d1\u5b66\u9662\u751f\u6001\u73af\u5883\u7814\u7a76\u4e2d\u5fc3\u57ce\u5e02\u4e0e\u533a\u57df\u751f\u6001\u56fd\u5bb6\u91cd\u70b9\u5b9e\u9a8c\u5ba4(State Key Lab of Urban and Regional Ecology, Research Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 10085, China)<\/p>\n

h<\/sup> \u4e2d\u56fd\u79d1\u5b66\u9662\u5927\u5b66(University of Chinese Academy of Sciences, Beijing , China)<\/p>\n

\u5199\u5728\u524d\u9762<\/h3>\n

\u5206\u4eab\u6807\u9898\uff1aEI\uff1a\u519c\u4e1a\u571f\u58e4\u5fae\u751f\u7269\u6838\u5fc3\u83cc\u7fa4\u80fd\u591f\u63d0\u5347\u571f\u58e4\u751f\u6001\u529f\u80fd<\/p>\n

\u5173\u952e\u5b57\uff1a\u5fae\u751f\u7269\u7f51\u7edc\uff0c\u6838\u5fc3\u8282\u70b9\uff0c\u529f\u80fd\u57fa\u56e0\uff0c\u771f\u83cc\uff0c\u7ec6\u83cc<\/p>\n

\u6458\u8981<\/h3>\n

\u590d\u6742\u751f\u6001\u7f51\u7edc\u4e2d\u7684\u8282\u70b9(\u7c7b\u7fa4)\u53ef\u4ee5\u6839\u636e\u5b83\u4eec\u5728\u7f51\u7edc\u4e2d\u7684\u8fde\u901a\u6027\u6c34\u5e73\uff0c\u57fa\u4e8e\u5b83\u4eec\u7684\u5728\u7f51\u7edc\u4e2d\u7684\u91cd\u8981\u4f5c\u7528\u8fdb\u884c\u5206\u7c7b\u3002\u7406\u8bba\u4e0a\uff0c\u4e00\u4e2a\u751f\u6001\u7f51\u7edc\u5185\u9ad8\u5ea6\u76f8\u8fde\u7684\u7c7b\u7fa4(\u6838\u5fc3\u8282\u70b9\uff0ckinless hubs<\/strong>)\u6bd4\u5173\u8054\u8f83\u5c11\u7684\u7c7b\u7fa4(peripherals)\u80fd\u591f\u652f\u6301\u66f4\u9ad8\u6c34\u5e73\u7684\u751f\u6001\u7cfb\u7edf\u529f\u80fd\u3002\u7136\u800c\uff0c\u6838\u5fc3\u8282\u70b9\u5728\u8c03\u8282\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u529f\u80fd\u6f5c\u529b\u4e2d\u7684\u4f5c\u7528\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u5c1a\u672a\u5f97\u5230\u63a2\u7d22\u4e0e\u7814\u7a76<\/strong>\u3002\u672c\u7814\u7a76\u901a\u8fc7\u5bf9\u5177\u6709\u91cd\u8981\u751f\u6001\u529f\u80fd\u4e0e\u7ecf\u6d4e\u4ef7\u503c\u7684\u519c\u4e1a\u751f\u6001\u7cfb\u7edf(\u5c0f\u9ea6\u548c\u7389\u7c73\u8f6e\u4f5c)\u7684\u534e\u5317\u5e73\u539f243\u4e2a\u571f\u58e4\u6837\u672c\u8fdb\u884c\u5927\u89c4\u6a21\u8c03\u67e5\uff0c\u5efa\u7acb\u4e86\u571f\u58e4\u771f\u83cc\u548c\u7ec6\u83cc\u7c7b\u7fa4\u7684\u5173\u8054\u7f51\u7edc\uff0c\u53d1\u73b0\u751f\u6001\u7f51\u7edc\u4e2d\u6838\u5fc3\u8282\u70b9\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u571f\u58e4\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u5448\u663e\u8457\u6b63\u76f8\u5173\uff0c\u8fd9\u4e9b\u57fa\u56e0\u5305\u62ecC\u56fa\u5b9a\u3001C\u964d\u89e3\u3001C\u7532\u9187\u3001N\u5faa\u73af\u3001P\u5faa\u73af\u548cS\u5faa\u73af\u7b49\u57fa\u56e0<\/strong>\u3002\u7ed3\u6784\u65b9\u7a0b\u6a21\u578b\u5206\u6790\u8fdb\u4e00\u6b65\u8868\u660e\uff0c\u6838\u5fc3\u8282\u70b9\uff0c\u800c\u4e0d\u662f\u6a21\u5757\u6216\u7f51\u7edc\u8282\u70b9\u7c7b\u7fa4\uff0c\u4e0e\u591a\u79cd\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u6709\u76f4\u63a5\u663e\u8457\u7684\u6b63\u76f8\u5173\u5173\u7cfb<\/strong>\u3002\u6211\u4eec\u7684\u7ed3\u679c\u8868\u660e\uff0c\u5728\u5fae\u751f\u7269\u7f51\u7edc\u4e2d\u88ab\u5f52\u7c7b\u4e3a\u6838\u5fc3\u8282\u70b9(kinless hubs)\u7684\u571f\u58e4\u5fae\u751f\u7269\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u571f\u58e4\u529f\u80fd\u6f5c\u529b\u9ad8\u5ea6\u76f8\u5173\uff0c\u8fd9\u5bf9\u4e8e\u5728\u5927\u7a7a\u95f4\u5c3a\u5ea6\u4e0a\u7406\u89e3\u548c\u7ba1\u7406(\u901a\u8fc7\u64cd\u7eb5\u5fae\u751f\u7269\u5173\u952e\u7269\u79cd)\u519c\u4e1a\u751f\u6001\u7cfb\u7edf\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002<\/p>\n

\u80cc\u666f<\/h3>\n

\u571f\u58e4\u5fae\u751f\u7269\u5171\u540c\u751f\u6d3b\u5728\u751f\u6001\u7f51\u7edc\u4e2d\uff0c\u5f62\u6210\u751f\u6001\u7c07\uff0c\u5f7c\u6b64\u5f3a\u70c8\u5171\u5b58\u3002\u8fd9\u4e9b\u751f\u6001\u7c07\u4e2d\u7684\u7c7b\u7fa4\u662f\u91cd\u8981\u7684\u751f\u6001\u5355\u5143\uff0c\u5177\u6709\u5171\u540c\u7684\u73af\u5883\u504f\u597d\uff0c\u4f46\u5728\u751f\u6001\u7c07\u5185\u548c\u751f\u6001\u7c07\u95f4\u7684\u8fde\u901a\u6027\u6c34\u5e73\u4e0a\u53ef\u80fd\u4e0e\u5176\u4ed6\u7c7b\u7fa4\u4e0d\u540c\u3002\u7b80\u5355\u5730\u8bf4\uff0c\u6709\u4e9b\u7c7b\u7fa4\u5728\u751f\u6001\u7c07(provincial hubs)\u6216\u751f\u6001\u7c07\u95f4(kinless hubs)\u9ad8\u5ea6\u76f8\u8fde\uff0c\u800c\u5176\u4ed6\u7c7b\u7fa4\u76f8\u5173\u7684\u7f51\u7edc\u8fde\u63a5\u6570\u91cf\u8981\u5c11\u5f97\u591a(\u5982peripheral and connector nodes)\u3002\u4e0e\u5176\u4ed6\u8282\u70b9\u4e0d\u540c\uff0cKinless hubs\u5728\u751f\u6001\u7f51\u7edc\u7684\u7ed3\u6784\u4e2d\u81f3\u5173\u91cd\u8981\uff0c\u53ef\u4ee5\u4e3a\u5176\u4ed6\u7c7b\u7fa4\u521b\u9020\u751f\u6001\u4f4d<\/strong>\u3002<\/p>\n

\u5728\u690d\u7269\u7fa4\u843d\u4e2d\uff0c\u5df2\u6709\u7814\u7a76\u53d1\u73b0Kinless hubs\u7684\u76f8\u5bf9\u4e30\u5ea6\u5bf9\u751f\u6001\u7cfb\u7edf\u529f\u80fd\u7684\u91cd\u8981\u6027\u3002\u5fae\u751f\u7269Kinless hubs<\/strong>\u53ef\u80fd\u62e5\u6709\u66f4\u52a0\u91cd\u8981\u7684\u529f\u80fd\u5c5e\u6027\uff0c\u5982\u517b\u5206\u5faa\u73af\u3001\u78b3\u5faa\u73af\u4ee5\u53ca\u6c2e\u56fa\u5b9a\u7b49\u3002\u8fd9\u4e9b\u8282\u70b9\u4e4b\u524d\u88ab\u5b9a\u4e49\u4e3a\u690d\u7269\u7fa4\u843d\u7684\u5173\u952e\u7269\u79cd<\/strong>\uff0c\u5b83\u4eec\u5728\u80fd\u91cf\u548c\u7269\u8d28\u6d41\u52a8\u4e2d\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\uff0c\u4e3a\u9646\u5730\u751f\u6001\u7cfb\u7edf\u63d0\u4f9b\u751f\u4ea7\u52a8\u529b\u3002\u8fd9\u4e9b\u7269\u79cd\u53ef\u80fd\u4e5f\u4ee3\u8868\u4e86\u5177\u6709\u6709\u9650\u529f\u80fd\u7684\u751f\u7269\u4f53\uff0c\u8fd9\u4e9b\u751f\u7269\u4f53\u4f9d\u8d56\u4e8e\u5b83\u4eec\u6240\u5173\u8054\u7684\u751f\u6001\u7c07\u6267\u884c\u7684\u751f\u6001\u529f\u80fd\u3002\u7136\u800c\uff0c\u76ee\u524d\u652f\u6301\u5fae\u751f\u7269\u7f51\u7edc\u4e2dkinless hubs\u76f8\u5bf9\u4e30\u5ea6\u91cd\u8981\u6027\u7684\u7814\u7a76\u8fd8\u8f83\u5c11\u3002\u4e3a\u4e86\u66f4\u597d\u5730\u4e86\u89e3\u9646\u5730\u751f\u6001\u7cfb\u7edf\u4e2d\u5fae\u751f\u7269\u7fa4\u843d\u5982\u4f55\u652f\u6301\u571f\u58e4\u529f\u80fd\uff0c\u786e\u5b9akinless hubs\u5fae\u751f\u7269\u7c7b\u7fa4\u7684\u73af\u5883\u504f\u597d\u548c\u529f\u80fd\u91cd\u8981\u6027\u663e\u5f97\u5c24\u4e3a\u91cd\u8981\u3002<\/p>\n

\u4e0e\u5df2\u62a5\u9053\u7684\u690d\u7269\u76f8\u4f3c\uff0c\u6211\u4eec\u5047\u8bbe\u751f\u6001\u7c07\u5185\u548c\u751f\u6001\u7c07\u95f4\u9ad8\u5ea6\u8fde\u63a5\u7684\u5fae\u751f\u7269\u7c7b\u7fa4(kinless hubs)\u53ef\u80fd\u4e0e\u6d89\u53ca\u591a\u4e2a\u751f\u6001\u7cfb\u7edf\u8fc7\u7a0b\u7684\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u5448\u6b63\u76f8\u5173\uff0c\u5305\u62ec\u78b3\uff0c\u6c2e\uff0c\u78f7\u548c\u786b\u7684\u5faa\u73af\u57fa\u56e0\u3002\u8fd9\u4e9b\u529f\u80fd\u5bf9\u4e8e\u7ef4\u6301\u690d\u7269\u751f\u4ea7\u975e\u5e38\u91cd\u8981<\/strong>\u3002\u56e0\u6b64\uff0c\u6211\u4eec\u5047\u8bbe\uff0ckinless hubs\u7684\u4e30\u5ea6\u5bf9\u4e8e\u7ef4\u6301\u4eba\u7c7b\u7ba1\u7406\u7684\u751f\u6001\u7cfb\u7edf\u7684\u751f\u4ea7\u529b\u81f3\u5173\u91cd\u8981<\/strong>\u3002<\/p>\n

\u4e3a\u4e86\u9a8c\u8bc1\u8fd9\u4e00\u5047\u8bbe\uff0c\u6211\u4eec\u5927\u5c3a\u5ea6(>1000 km)\u5206\u6790\u4e86\u534e\u5317\u5e73\u539f243\u4e2a\u571f\u58e4\u6837\u672c\u3002\u4e0a\u4e2a\u4e16\u7eaa\u4ee5\u6765\uff0c\u8be5\u5730\u533a\u4e00\u76f4\u8fdb\u884c\u5c0f\u9ea6-\u7389\u7c73\u53cc\u5b63\u8f6e\u4f5c\u96c6\u7ea6\u5316\u7ba1\u7406\uff0c\u7cae\u98df\u6d88\u8d39\u63d0\u4f9b\u5360\u4e2d\u56fd\u8c37\u7269\u603b\u4ea7\u91cf\u768450\uff05\u4ee5\u4e0a\u3002\u6700\u8fd1\u7684\u7814\u7a76\u8868\u660e\uff0c\u5bf9\u519c\u4f5c\u7269\u751f\u957f\u81f3\u5173\u91cd\u8981\u7684\u591a\u79cd\u571f\u58e4\u751f\u6001\u8fc7\u7a0b(\u4f8b\u5982\u517b\u5206\u5faa\u73af)\u662f\u7531\u571f\u58e4\u5fae\u751f\u7269\u7684\u529f\u80fd\u7279\u6027\u9a71\u52a8\u7684<\/strong>\uff0c\u56e0\u6b64\u901a\u8fc7\u8c03\u8282\u571f\u58e4\u5fae\u751f\u7269\u53ef\u4ee5\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u63d0\u9ad8\u519c\u4e1a\u751f\u4ea7\u529b\u548c\u53ef\u6301\u7eed\u6027\u3002\u7136\u800c\uff0c\u751f\u6001\u7f51\u7edc\u5185\u7684\u5fae\u751f\u7269\u7c7b\u7fa4\u5bf9\u4e8e\u7ef4\u6301\u8fd9\u4e9b\u751f\u6001\u7cfb\u7edf\u4e2d\u6f5c\u5728\u529f\u80fd\u7684\u91cd\u8981\u6027\u4ecd\u4e0d\u6e05\u695a\u3002\u4e8e\u662f\u6211\u4eec\u8fdb\u884c\u4e86qPCR\u5206\u6790\uff0c\u4ee5\u8ba1\u7b97\u53c2\u4e0eC\uff0cN\uff0cP\u548cS\u5faa\u73af\u7684\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\uff1b\u5e76\u5bf916Sr RNA\u548cITS\u6269\u589e\u5b50\u8fdb\u884c\u6d4b\u5e8f\uff0c\u5206\u6790\u76f8\u5173\u7ec6\u83cc\u548c\u771f\u83cc\u7684\u591a\u6837\u6027\u548c\u7fa4\u843d\u7ec4\u6210\u3002<\/p>\n

\u7ed3\u679c<\/h3>\n

\u771f\u83cc\u7fa4\u843d\u53ca\u529f\u80fd\u57fa\u56e0\u5206\u6790<\/h4>\n

Shi\u7b49\u4eba\u4e8e2018\u5e74\u5206\u6790\u4e86\u53d6\u6837\u5730\u571f\u58e4\u7ec6\u83cc\u7684\u591a\u6837\u6027\u548c\u7fa4\u843d\u7ec4\u6210\u3002\u4ece243\u4e2a\u571f\u58e4\u6837\u672c\u768415 184 073\u4e2a\u9ad8\u8d28\u91cf\u5e8f\u5217\u4e2d\u603b\u5171\u9274\u5b9a\u4e86208\u4e2a\u7ec6\u83ccOTU(97\uff05\u76f8\u4f3c\u6027)\u3002\u5728\u95e8\u6216\u7eb2\u6c34\u5e73\u4e0a\u4ee5\u653e\u7ebf\u83cc\u95e8\uff0c\u03b1\u53d8\u5f62\u83cc\u95e8\uff0c\u9178\u6746\u83cc\u95e8\uff0c\u03b3\u53d8\u5f62\u83cc\u95e8\uff0c\u03b2\u53d8\u5f62\u83cc\u95e8\u548c\u7eff\u6e7e\u83cc\u95e8\u7b49\u7c7b\u7fa4\u5360\u4f18\u52bf\uff0c\u5360\u603b\u5e8f\u5217\u768475%\u4ee5\u4e0a\u3002\u7ec6\u83cc\u7fa4\u843d\u7ec4\u6210\u4e0e\u571f\u58e4pH\u6709\u8f83\u5f3a\u7684\u76f8\u5173\u6027\u3002\u3010\u76f8\u5173\u6210\u679c\u53d1\u8868\u5728Spatial scale affects the relative role of stochasticity versus determinism in soil bacterial communities in wheat fields across the North China Plain. Microbiome 6,27.\u3011,\u4e2d\u6587\u89e3\u8bfb\u89c1 - Microbiome\uff1a\u7a7a\u95f4\u5c3a\u5ea6\u5bf9\u534e\u5317\u5e73\u539f\u9ea6\u7530\u571f\u58e4\u7ec6\u83cc\u7fa4\u843d\u7684\u5f71\u54cd(\u5357\u571f\u6240\u891a\u6d77\u71d5\u7ec4)\u3002<\/p>\n

\u4f7f\u7528Illumina\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\u6280\u672f\uff0c\u6211\u4eec\u83b7\u5f97\u4e86243\u4e2a\u571f\u58e4\u768411076\u4e2a\u771f\u83cc\u9ad8\u8d28\u91cf\u5e8f\u5217\uff0c\u9274\u5b9a\u51fa5068\u4e2aOTU (\u76f8\u4f3c\u6027\u4e3a97%)\u3002\u5728\u95e8\u6c34\u5e73\u4e0a\uff0c\u5b50\u56ca\u83cc\u95e8 (85.3%)\u3001\u62c5\u5b50\u83cc\u95e8 (6.0%)\u548c\u63a5\u5408\u83cc\u95e8(5.1%)\u4e3a\u4f18\u52bf\u79cd\uff0c\u5360\u603b\u5e8f\u5217\u768496%\u4ee5\u4e0a\u3002\u7ecfMantel\u68c0\u9a8c\u53d1\u73b0\uff0c\u571f\u58e4\u771f\u83cc\u7fa4\u843d\u7ec4\u6210\u4e0e\u571f\u58e4pH\u3001\u571f\u58e4\u6c34\u5206\u3001\u5168\u78f7\u3001\u5168\u6c2e\u5448\u6781\u663e\u8457\u6b63\u76f8\u5173\uff0c\u5176\u4e2d\u571f\u58e4pH\u5bf9\u771f\u83cc\u7fa4\u843d\u5f71\u54cd\u6700\u5927\u3002\u771f\u83cc\u03b1\u591a\u6837\u6027\u6307\u6570\u5982Simpson\u3001Shannon\u3001Chao1\u548cobserved species\u968f\u7740\u571f\u58e4DOC\u3001TN\u548cAP\u7684\u589e\u52a0\u800c\u663e\u8457\u964d\u4f4e\u3002\u57289\u4e2a\u6837\u5730\u768427\u4e2a\u534e\u5317\u5e73\u539f\u6837\u70b9\u4e2d\uff0c\u6211\u4eec\u5171\u68c0\u6d4b\u523071\u4e2a\u4e0e\u78b3(\u56fa\u5b9a)\u3001\u78b3(\u7532\u9187)\u3001\u78b3(\u964d\u89e3)\u3001\u6c2e\u3001\u78f7\u548c\u786b\u5faa\u73af\u76f8\u5173\u7684\u4e0d\u540c\u529f\u80fd\u57fa\u56e0\u3002\u7ecf\u8fc7Mantel\u68c0\u9a8c\uff0c\u53d1\u73b0\u571f\u58e4\u771f\u83cc\u7fa4\u843d\u7ec4\u6210\u4e0e\u571f\u58e4pH\u3001TP\u548cTK\u663e\u8457\u76f8\u5173\u3002\u5927\u591a\u6570\u529f\u80fd\u57fa\u56e0\u4e0e\u571f\u58e4pH\u3001TK\u5448\u663e\u8457\u6b63\u76f8\u5173\uff0c\u4e0eDTN\u3001DON\u5448\u663e\u8457\u8d1f\u76f8\u5173\u3002<\/p>\n

\u7f51\u7edc\u5206\u6790<\/h4>\n

\u5728\u76f8\u5173\u7f51\u7edc\u5206\u6790\u4e2d\u786e\u5b9a\u4e86\u516b\u4e2a\u751f\u6001\u7c07\u3002\u5728\u751f\u6001\u7f51\u7edc\u4e2d\uff0c\u5927\u591a\u6570\u5fae\u751f\u7269(\u8282\u70b9)(94%)\u88ab\u5f52\u7c7b\u4e3aperipherals\uff0c3%\u88ab\u5f52\u7c7b\u4e3aconnector hubs\uff0c3%\u88ab\u5f52\u7c7b\u4e3aprovincial hubs\uff0c\u53ea\u67090.2%\u88ab\u5f52\u7c7b\u4e3akinless hubs (\u56fe1A<\/strong>)\u3002\u8fd9\u4e9b\u7f51\u7edc\u8282\u70b9\u89d2\u8272\u4e0d\u5747\u5300\u5730\u5206\u5e03\u5728\u5df2\u8bc6\u522b\u7684\u751f\u6001\u7c07\u4e0a\u3002kinless hubs (\u4e24\u4e2a\u7cfb\u7edf\u578b)\u90fd\u5728module 7\u4e2d(\u56fe1B<\/strong>)\uff0c\u5212\u5206\u4e3a\u7ec6\u83cc\u7c7b\u7fa4\u3002\u8fd9\u4e24\u4e2a\u76f8\u5bf9\u7f55\u89c1\u7684kinless hubs\u5360\u603breads\u76840.17%\u3002kinless hubs\u5305\u62ec\u6765\u81ea\u82bd\u5355\u80de\u83cc\u95e8\u548cPhycisphaerae\u7684\u7cfb\u7edf\u578b\u3002\u5927\u591a\u6570connector\u4f4d\u4e8emodule 6\u548cmodule 8\u4e2d\uff0c\u5305\u62ec\u53d8\u5f62\u83cc\u95e8\u3001\u653e\u7ebf\u83cc\u95e8\u3001\u9178\u6746\u83cc\u95e8\u3001\u62df\u6746\u83cc\u95e8\u3001\u75a3\u5fae\u83cc\u9580\u3001\u63a5\u5408\u83cc\u95e8\u548c\u5b50\u56ca\u83cc\u95e8\u3002Provincial hubs\u5206\u5e03\u5728module 11\u3001 module 2\u4e0emodule 4\u3002<\/p>\n

\u56fe 1 \u57fa\u4e8e\u7f51\u7edc\u89d2\u8272\u7684\u7ec6\u83cc\u548c\u771f\u83ccOTU\u7684\u5206\u5e03\u56fe<\/h4>\n

\"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790<\/p>\n

\n

A<\/strong> \u57fa\u4e8e\u7f51\u7edc\u89d2\u8272\u7684\u7ec6\u83cc\u548c\u771f\u83ccOTU\u7684\u5206\u5e03\u56fe\u3002\u7f51\u7edc\u4e2d\u7684\u8282\u70b9\u6839\u636e\u5176\u5728\u7f51\u7edc\u4e2d\u7684\u89d2\u8272\u5206\u4e3aperipherals, modular hubs, network hubs \u6216connectors;<\/p>\n

B<\/strong> peripherals (\u7d2b\u8272), provincial hubs (\u7eff\u8272), kinless hubs (\u84dd\u8272) and connectors (\u7ea2\u8272) \u7684\u76f8\u5bf9\u4e30\u5ea6.<\/p>\n<\/blockquote>\n

\u8282\u70b9\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u529f\u80fd\u57fa\u56e0\u4e4b\u95f4\u7684\u8054\u7cfb<\/h4>\n

\u57fa\u4e8e\u5b9e\u9a8c\u6570\u636e\uff0c\u6211\u4eec\u7684\u7814\u7a76\u8868\u660e\uff1a\u751f\u6001\u7f51\u7edc\u4e2dkinless-hubs\u7269\u79cd\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u53c2\u4e0eC\u3001N\u3001P\u548cS\u5faa\u73af\u7684\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u5448\u663e\u8457\u6b63\u76f8\u5173\uff0c\u5176\u4e2d\u5305\u62ecC\u56fa\u5b9a\u3001C\u964d\u89e3\u3001C\u7532\u9187\u3001N\u5faa\u73af\u3001P\u5faa\u73af\u548cS\u5faa\u73af\u57fa\u56e0(\u56fe2<\/strong>\uff0c\u88681<\/strong>)\u3002Connector-hubs\u7269\u79cd\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u571f\u58e4\u529f\u80fd\u57fa\u56e0\u4e30\u5ea6\u5448\u5f31\u6b63\u76f8\u5173\u3002\u76f8\u53cd\uff0c\u88ab\u5f52\u7c7b\u4e3a\u6a21\u5757\u4e2d\u5fc3\u548cperipherals\u7684\u7269\u79cd\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u8fd9\u4e9b\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u5448\u8d1f\u76f8\u5173\u3002<\/p>\n

\u56fe 2 Kinless-hub OTUs \u7684\u5e73\u5747\u6807\u51c6\u5316\u4e30\u5ea6\u4e0e\u529f\u80fd\u57fa\u56e0\u4e30\u5ea6\u4e4b\u95f4\u7684\u5173\u7cfb<\/h4>\n

\"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790<\/p>\n

\n

Kinless-hub OTUs (\u7f51\u7edc\u8282\u70b9\uff1aOTU\uff0cOTU)\u7684\u5e73\u5747\u6807\u51c6\u5316\u4e30\u5ea6\u4e0e\u529f\u80fd\u57fa\u56e0\u4e30\u5ea6\u4e4b\u95f4\u7684\u5173\u7cfb(OTU\u548c\u57fa\u56e0\u5747\u7ecf\u8fc7lg\u8f6c\u5316)\uff0c\u6309C\u964d\u89e3(\u78b3\u964d\u89e3)\u3001C<\/em>\u56fa\u5b9a(\u78b3\u56fa\u5b9a)\u3001C_\u7532\u9187(\u78b3\u7532\u9187)\u3001N(\u6c2e\u5faa\u73af)\u3001P(\u78f7\u5faa\u73af)\u548cS(\u786b\u5faa\u73af)\u8fdb\u884c\u5206\u7ec4\u3002<\/p>\n<\/blockquote>\n

\u8868 1 kinless hubs, connectors, provincial hubs, peripherals\u4e0e\u571f\u58e4\u56e0\u5b50\u4e4b\u95f4\u76f8\u5173\u6027\u5f3a\u5ea6\u7684\u70ed\u56fe<\/h4>\n

\"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790<\/p>\n

\n

\u5728kinless hubs, connectors, provincial hubs, peripherals\u4e0e\u571f\u58e4\u56e0\u5b50\u4e4b\u95f4\u76f8\u5173\u6027\u5f3a\u5ea6\u7684\u70ed\u56fe\u3002\u6df1\u8272(\u84dd\u8272\u4e3a\u8d1f\uff0c\u7ea2\u8272\u4e3a\u6b63)\u8868\u793a\u8f83\u5f3a\u7684\u76f8\u5173\u6027\uff1b\u4ec5\u663e\u8457\u76f8\u5173(P <0.05)\u7684\u76f8\u5173\u7cfb\u6570(r)\u88ab\u8868\u793a\u51fa\u6765\u3002<\/p>\n<\/blockquote>\n

\u5728\u540c\u65f6\u8003\u8651\u571f\u58e4\u53d8\u91cf\u540e\uff0c\u7ed3\u6784\u65b9\u7a0b\u6a21\u578b\u8fdb\u4e00\u6b65\u8bc1\u660e\u4e86\u6bcf\u4e2a\u7f51\u7edc\u89d2\u8272\u4e2d\u7684\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u529f\u80fd\u57fa\u56e0\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e4b\u95f4\u7684\u5173\u7cfb(\u88681<\/strong>)\u3002\u7ed3\u6784\u65b9\u7a0b\u6a21\u578b\u8bc1\u5b9e\uff0ckinless-hubs\u7269\u79cd\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u591a\u529f\u80fd\u57fa\u56e0\u4e30\u5ea6\u5448\u6b63\u76f8\u5173(\u03b2=0.32\uff0c\u6807\u51c6\u5316\u56de\u5f52\u6743\u91cd)(\u56fe3A<\/strong>)\uff0cprovincial-hubs and peripherals\u7269\u79cd\u4e0e\u529f\u80fd\u57fa\u56e0\u4e30\u5ea6\u5448\u663e\u8457\u8d1f\u76f8\u5173(\u03b2=-0.27\u548c-0.26\uff0c\u6807\u51c6\u5316\u56de\u5f52\u6743\u91cd)(\u56fe3C\u548cD<\/strong>)\u3002connector-hubs\u7269\u79cd\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u571f\u58e4\u529f\u80fd\u7684\u76f8\u5173\u6027\u5f88\u5f31(\u03b2=0.0 1\uff0c\u6807\u51c6\u5316\u56de\u5f52\u6743\u91cd)(\u56fe3B<\/strong>)\u3002\u4e0d\u540c\u7f51\u7edc\u89d2\u8272\u7684\u7269\u79cd\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u4e0d\u540c\u7684\u571f\u58e4\u6027\u8d28\u6709\u5173\u3002\u4f8b\u5982\uff0cpH\u3001\u5168P\u3001\u5168K\u3001Ca\u548cCd\u5bf9kinless hubs\u6709\u76f4\u63a5\u7684\u6b63\u76f8\u5173(\u56fe3A<\/strong>)\u3002<\/p>\n

\u571f\u58e4AP\u548cMg\u5bf9provincial hubs\u6709\u5f3a\u70c8\u7684\u6b63\u76f8\u5173(\u56fe3C<\/strong>)\uff1bconnector-hubs\u7269\u79cd\u7684\u4e30\u5ea6\u4e0e\u571f\u58e4pH\u548c\u94dc\u5448\u6b63\u76f8\u5173(\u56fe3B<\/strong>)\uff1b\u800c\u51e0\u4e4e\u6ca1\u6709\u56e0\u7d20\u6b63\u76f8\u5173peripherals (\u56fe3D<\/strong>)\u3002\u571f\u58e4\u53d8\u91cf\u5bf9\u8282\u70b9\u548c\u529f\u80fd\u57fa\u56e0\u6709\u4e0d\u540c\u7684\u5f71\u54cd\uff0c\u4f46pH\u5bf9\u6240\u6709\u8282\u70b9\u548c\u529f\u80fd\u57fa\u56e0\u90fd\u6709\u76f4\u63a5\u5f71\u54cd\uff0c\u5982\u5bf9kinless , connector hubs\u53ca\u529f\u80fd\u57fa\u56e0\u6709\u6b63\u6548\u5e94\uff0c\u5bf9provincial,  peripheral hubs\u6709\u8d1f\u6548\u5e94\u3002<\/p>\n

\u56fe 3 \u571f\u58e4\u53d8\u91cf\u4e0e\u529f\u80fd\u57fa\u56e0\u4e30\u5ea6kinless hubs, connector hubs, provincial hubs\u548cperipheral hubs\u5173\u7cfb\u7684\u7ed3\u6784\u65b9\u7a0b\u6a21\u578b<\/h4>\n

\"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790<\/p>\n

\n

\u571f\u58e4\u53d8\u91cf\u4e0e\u529f\u80fd\u57fa\u56e0\u4e30\u5ea6kinless hubs(A),connector hubs (B), provincial hubs(C) and peripheral hubs (D)\u5173\u7cfb\u7684\u7ed3\u6784\u65b9\u7a0b\u6a21\u578b\u3002\u5b9e\u5fc3\u767d\u8272\u7bad\u5934\u4ee3\u8868\u663e\u8457\u6b63\u76f8\u5173(P<\/em><0.05)\uff0c\u865a\u767d\u8272\u7bad\u5934\u4ee3\u8868\u663e\u8457\u8d1f\u76f8\u5173(*P*<0.05)\uff0c\u5b9e\u5fc3\u7070\u8272\u7bad\u5934\u4ee3\u8868\u4e0d\u663e\u8457\u6b63\u76f8\u5173(*P*>0.05)\uff0c\u865a\u7070\u8272\u7bad\u5934\u4ee3\u8868\u4e0d\u663e\u8457\u8d1f\u76f8\u5173(P<\/em>>0.05)\u3002Cd\uff0c\u9549\uff1bK\uff0c\u94be\uff1bCa\uff0c\u9499\uff1bTK\uff0c\u5168\u94be\uff1bTP\uff0c\u603b\u78f7\uff1bMg\uff0c\u9541\uff1bCu\uff0c\u94dc\uff1bAP\uff0c\u6709\u6548\u78f7\uff1bDOC\uff0c\u53ef\u6eb6\u6027\u6709\u673a\u78b3\uff1bFe\uff0c\u94c1\uff1bMn\uff0c\u9530\uff1bAs\uff0c\u7837\u3002\u9ec4\u70b9\u548c\u7ea2\u70b9\u662f\u7f51\u7edc\u4e2d\u7684\u8282\u70b9\u3002\u9ec4\u70b9\u662f\u6a21\u5757\u4e2d\u7684\u516c\u5171\u8282\u70b9\uff0c\u7ea2\u70b9\u662f\u6a21\u5757\u4e2d\u7684\u4e2d\u5fc3\u8282\u70b9\u3002(\u8981\u89e3\u91ca\u6b64\u56fe\u4f8b\u4e2d\u5bf9\u989c\u8272\u7684\u5f15\u7528\uff0c\u8bf7\u53c2\u9605\u672c\u6587\u7684\u7f51\u7edc\u7248\u672c\u3002)<\/p>\n<\/blockquote>\n

\u8ba8\u8bba<\/h3>\n

\u6211\u4eec\u7684\u7814\u7a76\u53d1\u73b0\u7c7b\u4f3c\u4e8e\u690d\u7269\u7fa4\u843d\uff0c\u751f\u6001\u7f51\u7edc\u4e2d\u88ab\u5f52\u7c7b\u4e3akinless-hubs\u5fae\u751f\u7269\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u9ad8\u6c34\u5e73\u7684\u529f\u80fd\u6f5c\u529b\u6709\u5173\u3002Connector-hubs\u7269\u79cd\u7684\u76f8\u5bf9\u4e30\u5ea6\u5bf9\u7ef4\u6301\u7f51\u7edc\u4e2d\u7684\u591a\u79cd\u529f\u80fd\u57fa\u56e0\u4e5f\u5f88\u91cd\u8981<\/strong>\uff0c\u4f46peripheral\u548c provincial\u7269\u79cd\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u53c2\u4e0eC\u3001N\u3001P\u548cS\u5faa\u73af\u7684\u529f\u80fd\u57fa\u56e0\u4e30\u5ea6\u5448\u663e\u8457\u8d1f\u76f8\u5173\u3002\u5373\u4f7f\u5728\u8003\u8651\u4e86\u591a\u79cd\u571f\u58e4\u6027\u8d28\u540e\uff0c\u8fd9\u4e9b\u7ed3\u679c\u4ecd\u7136\u6709\u6548\u3002\u6211\u4eec\u7684\u7814\u7a76\u8868\u660e\uff0c\u76f8\u5173\u7f51\u7edc\u4e2d\u76f8\u5bf9\u7f55\u89c1\u4f46\u9ad8\u5ea6\u5173\u8054\u7684\u7c7b\u7fa4\u4e0e\u53c2\u4e0eC\u3001N\u3001P\u548cS\u5faa\u73af\u7684\u91cd\u8981\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u5448\u663e\u8457\u6b63\u76f8\u5173<\/strong>\u3002\u5728\u5229\u7528\u7ed3\u6784\u65b9\u7a0b\u6a21\u578b\u540c\u65f6\u8003\u8651\u591a\u79cd\u571f\u58e4\u6027\u8d28\u7684\u5f71\u54cd\u7684\u540e\uff0c\u8fd9\u4e9b\u7ed3\u679c\u4ecd\u7136\u6210\u7acb\uff0c\u5373kinless taxa\u4e30\u5ea6\u9ad8\u7684\u5730\u70b9\u4e0e\u9ad8\u529f\u80fd\u6f5c\u529b\u5448\u6b63\u76f8\u5173\u3002\u6211\u4eec\u7684\u7ed3\u679c\u8868\u660e\uff0c\u5728\u751f\u6001\u7f51\u7edc\u4e2d\u626e\u6f14\u4e0d\u540c\u89d2\u8272\u7684\u7c7b\u7fa4\u5728\u4e0d\u540c\u7684\u73af\u5883\u68af\u5ea6\u4e0a\u5bf9\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u6709\u4e0d\u540c\u7684\u5f71\u54cd<\/strong>\u3002<\/p>\n

kinless taxa\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u5448\u6b63\u76f8\u5173<\/h4>\n

\u6211\u4eec\u91cf\u5316\u4e86\u534e\u5317\u5e73\u539f27\u4e2a\u6837\u70b9\u4e2d\u53c2\u4e0eC\u3001N\u3001S\u548cP\u5faa\u73af\u768471\u4e2a\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u3002\u8d85\u8fc790%\u7684\u529f\u80fd\u57fa\u56e0\u4e0e\u4e24\u4e2a\u5df2\u9274\u5b9a\u7684kinless hubs\u7684\u76f8\u5bf9\u4e30\u5ea6\u5448\u6b63\u76f8\u5173\uff0c\u7ea670%\u7684\u57fa\u56e0\u4e0econnector-hubs\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u5448\u6b63\u76f8\u5173\u3002\u56e0\u6b64\uff0ckinless\u548cconnector-hubs\u4f5c\u4e3a\u529f\u80fd\u6f5c\u529b\u7684\u8c03\u8282\u5668\u7684\u91cd\u8981\u6027\u53ef\u80fd\u4e0e\u5176\u5728\u7f51\u7edc\u4e2d\u7684\u62d3\u6251\u89d2\u8272\u6709\u5173<\/strong>\u3002Kinless\u548cConnector-Hub<\/strong>\u4e0e\u751f\u6001\u7c07\u5185\u548c\u751f\u6001\u7c07\u95f4\u7684\u5176\u4ed6\u7c7b\u7fa4\u76f8\u5173(C scores >0.62)\uff0c\u8868\u660e\u8fd9\u4e9b\u7c7b\u7fa4\u53ef\u4ee5\u901a\u8fc7\u4fc3\u8fdb\u517b\u5206\u4ea4\u6362\u548c\u8d44\u6e90\u53ef\u83b7\u5f97\u6027\uff0c\u5728\u7ef4\u6301\u591a\u79cd\u751f\u6001\u7cfb\u7edf\u8fc7\u7a0b\u4e2d\u53d1\u6325\u591a\u91cd\u4f5c\u7528<\/strong>\u3002<\/p>\n

\u6211\u4eec\u7684\u7ed3\u679c\u5f3a\u8c03\u4e86\u8fd9\u4e9b\u7c7b\u7fa4\u5bf9\u53c2\u4e0e\u8c03\u8282\u591a\u79cd\u751f\u6001\u7cfb\u7edf\u529f\u80fd\u7684\u591a\u79cd\u529f\u80fd\u57fa\u56e0\u7684\u91cd\u8981\u6027\uff0c\u8fd9\u4e9b\u529f\u80fd\u57fa\u56e0\u5305\u62ecC\u56fa\u5b9a\u3001N\u56fa\u5b9a\u3001P\u77ff\u5316\u548c\u51cb\u843d\u7269\u5206\u89e3\u3002Provincial- and Peripheral-\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u5448\u8d1f\u76f8\u5173\uff0c\u4e0eKinless-\u3001Connector- \u7c7b\u7fa4\u4e0d\u540c\uff0c\u8fd9\u8ddfDeng(2015)\u957f\u671f\u7530\u95f4\u8bd5\u9a8c\u7684\u7ed3\u679c\u4e2dprovincial hubs\u4e0eC\u5faa\u73af\u57fa\u56e0\u5448\u8d1f\u76f8\u5173\u4fdd\u6301\u4e00\u81f4\u3002Provincial hubs\u4e0e\u4e00\u4e2a\u6a21\u5757\u4e2d\u7684\u7c7b\u7fa4\u9ad8\u5ea6\u76f8\u5173\uff0c\u5728\u8fd9\u4e2a\u6a21\u5757\u91cc\uff0c\u5fae\u751f\u7269\u4e2a\u4f53\u5171\u4eab\u76f8\u4f3c\u7684\u751f\u6001\u4f4d\u6216\u6267\u884c\u76f8\u4f3c\u7684\u529f\u80fd\u3002\u5176\u4e2d\u9ad8\u4e30\u5ea6\u7684\u7c7b\u7fa4\u53ef\u80fd\u4e0e\u5176\u4ed6\u6a21\u5757\u4e2d\u7684\u6210\u5458\u5b58\u5728\u6fc0\u70c8\u7684\u7ade\u4e89\uff0c\u8fd9\u5bf9\u5f62\u6210\u5b8c\u6574\u751f\u6001\u7f51\u7edc\u53ef\u80fd\u6ca1\u6709\u5e2e\u52a9\u3002\u7531\u4e8e\u6574\u4e2a\u751f\u6001\u7f51\u7edc\u5305\u542b\u591a\u4e2a\u6a21\u5757\uff0c\u6267\u884c\u5404\u79cd\u529f\u80fd\uff0c\u4e00\u4e2a\u6a21\u5757\u4e2d\u67d0\u4e9b\u9ad8\u4e30\u5ea6\u7684\u7c7b\u7fa4\u53ef\u80fd\u4f1a\u5bf9\u751f\u6001\u7f51\u7edc\u7684\u7a33\u5b9a\u9020\u6210\u4e0d\u5229\u5f71\u54cd\u3002\u8fd9\u53ef\u80fd\u662f\u4e3a\u4ec0\u4e48\u6211\u4eec\u53d1\u73b0provincial hubs\u4e0e\u6700\u4e3b\u8981\u7684\u529f\u80fd\u57fa\u56e0\u5448\u8d1f\u76f8\u5173\uff0c\u800c\u6a21\u5757\u4e2d\u4e00\u4e9b\u9ad8\u4e30\u5ea6\u7684\u95e8\u4e0e\u529f\u80fd\u57fa\u56e0\u5448\u8d1f\u76f8\u5173\u7684\u539f\u56e0\u3002\u7c7b\u4f3c\u5730\uff0cperipherals\u7684\u9ad8\u4e30\u5ea6\u7c7b\u7fa4\u53ef\u80fd\u5f71\u54cd\u6574\u4e2a\u751f\u6001\u7f51\u7edc\u7684\u591a\u529f\u80fd\uff0c\u56e0\u6b64\u6211\u4eec\u53d1\u73b0\u4e24\u8005\u4e4b\u95f4\u4e5f\u5b58\u5728\u8d1f\u76f8\u5173\u5173\u7cfb\u3002<\/p>\n

\u5173\u952e\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u4e0e\u7a00\u6709\u4f46\u9ad8\u5ea6\u76f8\u8fde\u7684kinless and connector hubs\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e4b\u95f4\u7684\u663e\u8457\u6b63\u76f8\u5173\u6027\u8868\u660e\uff0c\u8fd9\u4e9b\u7269\u79cd\u53ef\u80fd\u5728\u7ef4\u6301\u5728\u5305\u62ec\u5c0f\u9ea6\u548c\u7389\u7c73\u79cd\u690d\u5728\u5185\u7684\u91cd\u8981\u519c\u4e1a\u751f\u6001\u7cfb\u7edf\u4e2d\u7684\u591a\u4e2a\u751f\u6001\u7cfb\u7edf\u8fc7\u7a0b\u7684\u529f\u80fd\u4e2d\u53d1\u6325\u91cd\u8981\u4f5c\u7528\u3002\u5c5e\u4e8e\u82bd\u5355\u80de\u83cc\u95e8\u548c\u566c\u83cc\u4f53\u7684kinless-hubs\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u529f\u80fd\u57fa\u56e0\u7684\u4e30\u5ea6\u6b63\u76f8\u5173\uff0c\u8fd9\u4e9b\u529f\u80fd\u57fa\u56e0\u5305\u62ecC\u56fa\u5b9a\uff0cC\u964d\u89e3\uff0cC\u7532\u9187\uff0cN\u5faa\u73af\uff0cP\u5faa\u73af\u548cS\u5faa\u73af\u3002\u4f8b\u5982\uff0cexoPG(\u679c\u80f6\u9176)\u3001pmoA(\u7532\u70f7\/\u6c28328\u5355\u52a0\u6c27\u9176\u4e9a\u5355\u4f4dA)\u548ckinless-hubs\u4e4b\u95f4\u7684\u6700\u5f3a\u6b63\u76f8\u5173\u8868\u660e\u53ef\u80fd\u6709\u5f88\u5f3a\u7684\u79ef\u7d2fN\u7684\u80fd\u529b\u3002\u82bd\u5355\u80de\u83cc\u95e8\u542b\u6709\u4ee5\u53f6\u7eff\u7d20\u4e3a\u57fa\u7840\u7684\u5149\u8425\u517b\u7269\u79cd\uff0c\u8fd9\u8868\u660e\u5176\u6709\u5f88\u5f3a\u7684\u80fd\u529b\u652f\u6301\u57fa\u672c\u7684\u751f\u7269\u8fc7\u7a0b\u3002\u82bd\u5355\u80de\u83cc\u95e8\u4e2d\u7684\u7c7b\u7fa4\u53ef\u4ee5\u9002\u5e94\u5e72\u71e5\u7684\u6761\u4ef6\u3002\u534e\u5317\u5e73\u539f\u603b\u4f53\u4e0a\u662f\u5e72\u65f1\u7684\uff0c\u8fdb\u4e00\u6b65\u8868\u660e\u8be5\u7c7b\u7fa4\u5728\u5168\u7403\u5360\u4e3b\u5bfc\u5730\u4f4d\u7684\u751f\u6001\u7cfb\u7edf\u4e2d\u53ef\u80fd\u5360\u6709\u91cd\u8981\u7684\u529f\u80fd\uff0c\u5982\u65f1\u5730\u7684\u751f\u6001\u7cfb\u7edf\u3002\u4f8b\u5982\uff0c\u82bd\u5355\u80de\u83cc\u95e8\u88ab\u9274\u5b9a\u4e3a\u534e\u5317\u5730\u533a\u70df\u8349\u571f\u58e4\u4e2d\u7684\u5173\u952e\u79cd\u3002\u53e6\u4e00\u4e2akinless hub\u5c5e\u4e8e\u6d6e\u9709\u83cc\u95e8\u7684Phycisphaerae\uff0c\u800c\u5728\u4e0d\u540c\u7684\u6816\u606f\u5730\u90fd\u53d1\u73b0\u4e86\u6d6e\u9709\u83cc\u95e8\u3002\u4f8b\u5982\uff0c\u4e00\u4e9b\u7269\u79cd\u5728\u538c\u6c27\u6761\u4ef6\u4e0b\u5177\u6709\u6c27\u5316\u94f5\u7684\u80fd\u529b\uff0c\u901a\u8fc7\u8fd9\u4e2a\u8fc7\u7a0b\uff0c\u8fd9\u4e9b\u7269\u79cd\u53ef\u4ee5\u751f\u6d3b\u5728\u6e29\u6cc9\u4e2d\u6216\u5357\u6781\u6c34\u57df\u3002\u6b64\u5916\uff0c\u6d6e\u9709\u83cc\u95e8\u7ecf\u5e38\u4e0e\u690d\u7269\u6839\u9645\u8054\u7cfb\u5728\u4e00\u8d77\uff0c\u5982\u68c9\u82b1\u3001\u91ce\u751f\u751c\u83dc\u548c\u51ac\u5c0f\u9ea6\u3002\u6839\u9645\u662f\u517b\u5206\u4ea4\u6362\u548c\u5143\u7d20\u5faa\u73af\u7684\u67a2\u7ebd\uff0c\u5e76\u751f\u6d3b\u7740\u6570\u4ee5\u767e\u4e07\u8ba1\u7684\u5fae\u751f\u7269\uff0c\u53d1\u6325\u8c03\u8282\u8425\u517b\u5faa\u73af\u6216\u6291\u5236\u75c5\u539f\u4f53\u7684\u91cd\u8981\u4f5c\u7528\u3002\u56e0\u6b64\uff0c\u6d6e\u9709\u83cc\u95e8\u53ef\u80fd\u901a\u8fc7\u53c2\u4e0e\u591a\u79cd\u751f\u6001\u8fc7\u7a0b\u95f4\u63a5\u4fc3\u8fdb\u690d\u7269\u751f\u957f\u3002\u672a\u6765\u7684\u7814\u7a76\u76ee\u6807\u5e94\u8be5\u662f\u57f9\u517b\u8fd9\u4e24\u4e2a\u5fae\u751f\u7269\u7c7b\u7fa4\uff0c\u5e76\u68c0\u6d4b\u5b83\u4eec\u5728\u63a7\u5236\u6761\u4ef6\u4e0b\u4fc3\u8fdb\u519c\u7530\u751f\u6001\u7cfb\u7edf\u529f\u80fd\u7684\u6f5c\u5728\u9002\u7528\u6027\u3002<\/p>\n

\u6211\u4eec\u8fd8\u53d1\u73b0connector-hubs\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u4e0e\u571f\u58e4\u529f\u80fd\u57fa\u56e0\u7684\u7edd\u5bf9\u4e30\u5ea6\u4e4b\u95f4\u5b58\u5728\u663e\u8457\u6b63\u76f8\u5173\u7684\u5173\u7cfb\u3002\u4e0d\u540c\u4e8ekinless\u7c7b\u7fa4\uff0cconnector\u7c7b\u7fa4\u65e2\u5305\u62ec\u7ec6\u83cc\uff0c\u4e5f\u5305\u62ec\u771f\u83cc\u3002\u5b50\u56ca\u83cc\u95e8Sordariomycetes\u7684\u4e00\u4e2a\u8fde\u63a5\u5b50\u4e0e\u5927\u591a\u6570\u57fa\u56e0\u5448\u6b63\u76f8\u5173\uff0cSordariomycetes\u53ef\u4ee5\u5206\u89e3\u51cb\u843d\u7269\u548c\u6728\u6750\uff0c\u5728\u5404\u79cd\u571f\u58e4\u751f\u5883\u4e2d\u666e\u904d\u5b58\u5728\u3002\u53e6\u4e00\u79cd\u75a3\u5fae\u83cc\u95e8\u8fde\u63a5\u5b50\u4e0e\u5927\u591a\u6570\u57fa\u56e0\u5448\u663e\u8457\u6b63\u76f8\u5173\uff0c\u800c\u5df2\u6709\u7814\u7a76\u53d1\u73b0\u75a3\u5fae\u83cc\u95e8\u7684\u4e30\u5ea6\u4e0eC\u5faa\u73af\u76f8\u5173\u7684\u5404\u79cd\u57fa\u56e0\u5448\u6b63\u76f8\u5173\u3002\u6211\u4eec\u7684\u7ed3\u679c\u63ed\u793a\u4e86\u5fae\u751f\u7269\u7f51\u7edc\u4e2d\u9ad8\u5ea6\u76f8\u8fde\u7684\u7c7b\u7fa4\u7684\u4e30\u5ea6\u4e0e\u571f\u58e4\u529f\u80fd\u57fa\u56e0\u4e4b\u95f4\u7684\u76f4\u63a5\u8054\u7cfb\uff0c\u8fd9\u8868\u660e\u7814\u7a76hub-related\u7269\u79cd\u7684\u4e30\u5ea6\u5c06\u4e3a\u7ef4\u6301\u6216\u6539\u5584\u519c\u4e1a\u751f\u6001\u7cfb\u7edf\u7684\u529f\u80fd\u63d0\u4f9b\u91cd\u8981\u4fe1\u606f<\/strong>\u3002<\/p>\n

\u571f\u58e4\u6027\u8d28\u5bf9kinless\u548cconnector\u7c7b\u7fa4\u76f8\u5bf9\u4e30\u5ea6\u7684\u5f71\u54cd<\/h4>\n

\u571f\u58e4\u6027\u8d28\u5bf9kinless \u548c connector\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u5f71\u54cd\u8f83\u5927\u3002\u4f8b\u5982\uff0cpH\u4e0ekinless\u548c connector hubs\u7684\u76f8\u5bf9\u4e30\u5ea6\u5448\u6b63\u76f8\u5173\uff0c\u800c\u4e0eprovincial \u548cperipheral hubs\u7684\u76f8\u5bf9\u4e30\u5ea6\u5448\u8d1f\u76f8\u5173\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0c\u65bd\u7528\u6709\u673a\u80a5\u6216\u77f3\u7070\u8c03\u8282pH\u53ef\u80fd\u901a\u8fc7\u589e\u52a0kinless\u548cconnector hubs\u7684\u4e30\u5ea6\uff0c\u964d\u4f4eprovincial\u548cperipheral hubs\u7684\u4e30\u5ea6\u6765\u6539\u5584\u571f\u58e4\u529f\u80fd<\/strong>\u3002\u8c03\u8282\u5176\u4ed6\u571f\u58e4\u53d8\u91cf\uff0c\u5982\u9499\u548c\u5168\u94be\uff0c\u4e5f\u53ef\u4ee5\u901a\u8fc7\u6539\u53d8\u7f51\u7edc\u4e2d\u8fd9\u4e9b\u67a2\u7ebd\u7684\u4e30\u5ea6\u6765\u6539\u5584\u571f\u58e4\u7684\u529f\u80fd\u3002\u5bf9\u4e8e\u91cd\u8981\u7684\u8c37\u7c7b\u4f5c\u7269\u5c0f\u9ea6\uff0c\u627e\u5230\u4e00\u79cd\u66f4\u52a0\u73af\u5883\u53cb\u597d\u7684\u65b9\u6cd5\u6765\u62b5\u5fa1\u75be\u75c5\u548c\u9002\u5e94\u6c14\u5019\u53d8\u5316\u662f\u81f3\u5173\u91cd\u8981\u7684\u3002\u6709\u7814\u7a76\u5efa\u8bae\u6700\u5927\u9650\u5ea6\u5730\u53d1\u6325\u519c\u4e1a\u751f\u6001\u7cfb\u7edf\u5fae\u751f\u7269\u7fa4\u843d\u7684\u6838\u5fc3\u529f\u80fd\u53ef\u80fd\u662f\u5e94\u5bf9\u519c\u4e1a\u751f\u6001\u7cfb\u7edf\u9000\u5316\u548c\u8d44\u6e90\u77ed\u7f3a\u7684\u6700\u4f73\u9009\u62e9<\/strong>\u3002\u4eca\u540e\u7684\u7814\u7a76\u5e94\u6df1\u5165\u4e86\u89e3\u571f\u58e4\u56e0\u5b50\u3001\u529f\u80fd\u57fa\u56e0\u548c\u5fae\u751f\u7269\u7fa4\u843d\u6838\u5fc3\u529f\u80fd\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u786e\u5b9a\u9ad8\u5ea6\u8054\u7cfb\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u662f\u5426\u53ef\u4ee5\u901a\u8fc7\u5404\u79cd\u519c\u4e1a\u5b9e\u8df5\u6765\u64cd\u7eb5\u5fae\u751f\u7269\u5173\u952e\u7269\u79cd\uff0c\u4ece\u800c\u51cf\u5c11\u571f\u58e4\u75c5\u5bb3\u548c\u63d0\u9ad8\u4f5c\u7269\u7684\u9002\u5e94\u6027\u3002<\/p>\n

\u7ed3\u8bba<\/h3>\n

\u7efc\u4e0a\u6240\u8ff0\uff0c\u6211\u4eec\u7684\u7814\u7a76\u7ed3\u679c\u8868\u660e\uff0c\u6570\u91cf\u8f83\u5c11\u7684\u9ad8\u8fde\u63a5\u5ea6\u7c7b\u7fa4\u7684\u76f8\u5bf9\u4e30\u5ea6\u5bf9\u4e8e\u4fdd\u6301\u8f83\u9ad8\u7684\u529f\u80fd\u6f5c\u529b\u81f3\u5173\u91cd\u8981\uff0c\u5bf9\u4e8e\u7406\u89e3\u5927\u578b\u7a7a\u95f4\u96c6\u7ea6\u5316\u519c\u4e1a\u751f\u6001\u7cfb\u7edf\u7684\u529f\u80fd\u5177\u6709\u91cd\u8981\u610f\u4e49<\/strong>\u3002\u8981\u5728\u571f\u58e4\u4e2d\u57f9\u517b\u6570\u4ee5\u5343\u8ba1\u7684\u5fae\u751f\u7269\u662f\u4e0d\u53ef\u884c\u7684\uff0c\u7136\u800c\uff0c\u805a\u7126\u672c\u7814\u7a76\u4e2d\u786e\u5b9a\u51cf\u5c11\u7684\u5173\u952e\u7c7b\u7fa4\u4e9a\u96c6\uff0c\u53ef\u80fd\u5bf9\u7ef4\u6301\u4eba\u7c7b\u7ba1\u7406\u7684\u751f\u6001\u7cfb\u7edf\u4e0b\u7684\u751f\u6001\u7cfb\u7edf\u529f\u80fd\u5177\u6709\u91cd\u8981\u610f\u4e49<\/strong>\u3002<\/p>\n

\u65b9\u6cd5\u4e0e\u6750\u6599<\/h3>\n

\u91c7\u6837\u5730\u3001DNA\u63d0\u53d6\u4e0e\u571f\u58e4\u53d8\u91cf<\/h4>\n

\u6211\u4eec\u4e8e2014\u5e7411\u670820\u65e5\u81f311\u670830\u65e5\u572827\u4e2a\u91c7\u6837\u70b9\u91c7\u96c6\u4e86243\u4e2a\u571f\u58e4\u6837\u54c1(\u56feS1A<\/strong>)\u6765\u7814\u7a76\u534e\u5317\u5e73\u539f\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u5206\u5e03\u3002\u8fd9\u4e00\u5730\u533a\u7684\u8f6e\u4f5c\u5236\u5ea6\u662f\u51ac\u5c0f\u9ea6\u548c\u590f\u7389\u7c73\u3002\u6837\u54c1\u91c7\u96c6\u7684\u8be6\u7ec6\u4fe1\u606f\u89c1(Shi et al. 2018 Microbiome)\u3002\u7b80\u800c\u8a00\u4e4b\uff0c\u5728\u6bcf\u4e2a\u5730\u70b9(100 km2<\/sup>)\u9009\u62e9\u4e869\u4e2a\u5730\u5757\uff0c\u4efb\u610f\u4e24\u4e2a\u5730\u5757\u4e4b\u95f4\u7684\u8ddd\u79bb\u4e3a3.3 km (\u56feS1B<\/strong>)\u3002\u6211\u4eec\u5728\u6bcf\u4e2a\u5730\u5757\u6536\u96c6\u4e8612\u4e2a\u70b9\uff0c\u6df1\u5ea6\u4e3a15 cm\uff0c\u5c06\u5b83\u4eec\u6df7\u5408\u5728\u4e00\u8d77\u4f5c\u4e3a\u4e00\u4e2a\u6837\u672c\uff0c\u6240\u6709\u7684243\u4e2a\u6837\u672c\u88ab\u4fdd\u5b58\u5728\u51b0\u76d2\u4e2d\uff0c\u5e76\u7acb\u5373\u8fd0\u5f80\u5b9e\u9a8c\u5ba4\u3002\u571f\u58e4DNA\u63d0\u53d6\u4e0e\u571f\u58e4\u53d8\u91cf\u7684\u6d4b\u91cf\u89c12018 Microbiome\u3002<\/p>\n

\"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790<\/p>\n

\n

\u56fe S1 \u9ea6\u7530\u571f\u58e4\u6837\u54c1\u91c7\u6837\u56fe<\/p>\n<\/blockquote>\n

\u6570\u636e\u5206\u6790<\/h4>\n

\u8be6\u7ec6\u9ad8\u901a\u91cf\u7ec6\u83ccDNA\u5206\u6790\u89c1Microbiome\uff1a\u7a7a\u95f4\u5c3a\u5ea6\u5bf9\u534e\u5317\u5e73\u539f\u9ea6\u7530\u571f\u58e4\u7ec6\u83cc\u7fa4\u843d\u7684\u5f71\u54cd\u3002<\/p>\n

\u4f7f\u7528\u5f15\u7269(515F\uff0c5\u2018-GTGCCAGCMGCCGCGGTAA-3\u2019\uff1b806R\uff0c5\u2018-GGACTACHVGGGGTWTCTAAT-3\u2019)\u7ed3\u5408\u9002\u914d\u5e8f\u5217\u548c\u6761\u5f62\u7801\u5e8f\u5217\uff0c\u6269\u589e\u7ec6\u83cc16Sr RNA  V4\u9ad8\u53d8\u533a\u3002\u4f7f\u7528\u5f15\u7269(ITS1-F\uff1aCTTGGTCATTTAGAGGAAGTAA\uff0cITS2-R\uff1aGCTGCGTCTTCATCGATGC)\u7ed3\u5408\u9002\u914d\u5e8f\u5217\u548c\u6761\u5f62\u7801\u5e8f\u5217\uff0c\u6269\u589e\u771f\u83ccITS1\u533a\u57df\u3002\u572850-\u03bcl\u53cd\u5e94\u4f53\u7cfb\u4e2d\u6269\u589e\u51fa\u6bcf\u4e2a\u6837\u54c1\u7684\u4e09\u8054\u4f53\uff0c\u6761\u4ef6\u4e3a\uff1a30\u4e2a\u5faa\u73af\uff0c94\u00b0C\u53d8\u6027\uff0c54\u00b0C\u9000\u706b30s\uff0c30\u00b0C\u5ef6\u4f381min\uff0c72\u00b0C\u5ef6\u4f387min\u3002\u5c06\u6837\u672c\u7684\u4e09\u91cdPCR\u4ea7\u7269\u6df7\u5408\u7528\u4e8e\u7ec6\u83cc\u548c\u771f\u83cc\u5206\u6790\uff0c\u4f7f\u7528QIA Quick 132 PCR\u7eaf\u5316\u8bd5\u5242\u76d2(Qiagen\uff0cHilden\uff0c\u5fb7\u56fd)\u7eaf\u5316\uff0c\u7136\u540e\u4f7f\u7528NanoDrop  ND-1000\u5206\u5149\u5149\u5ea6\u8ba1(Thermo Science\uff0cWaltham\uff0c\u7f8e\u56fd)\u8fdb\u884c\u5b9a\u91cf\u3002PCR\u4ea7\u7269\u7b49\u6469\u5c14\u6bd4\u6df7\u5408\u5728\u5355\u4e2a\u8bd5\u7ba1\u4e2d\uff0c\u4f7f\u7528Illumina MiSeq\u5e73\u53f0\u8fdb\u884c\u6d4b\u5e8f\u3002<\/p>\n

\u5728Wafergen Smart Chip Real-time qPCR\u5e73\u53f0\u4e0a\uff0c\u901a\u8fc7\u9ad8\u901a\u91cfqPCR\u5bf9243\u4e2aDNA\u6837\u672c\u8fdb\u884c71\u4e2a\u529f\u80fd\u57fa\u56e0(\u5982C\u3001N\u548cP\u5faa\u73af)\u5206\u6790\u3002\u6bcf\u4e2aDNA\u6837\u54c1\u7528PCR\u6269\u589e\uff0c\u4e00\u5f0f\u4e09\u4efd\uff0c\u68c0\u6d4b\u9650\u4e3a31\u4e2a\u9608\u503c\u5468\u671f\u3002\u5f53\u88ab\u591a\u4e2a\u5f15\u7269\u7ec4\u9776\u5411\u65f6\uff0c\u540c\u4e00\u57fa\u56e0\u88ab\u8ba4\u4e3a\u662f\u552f\u4e00\u7684\u72ec\u7279\u529f\u80fd\u3002\u6309\u7167Pfaffl\u7684\u65b9\u6cd5\u8ba1\u7b97\u6bcf\u4e2a\u6837\u672c\u7684\u529f\u80fd\u57fa\u56e0\u7684\u76f8\u5bf9\u4e30\u5ea6(\u57fa\u56e0\u62f7\u8d1d\u6807\u51c6\u5316\u4e3a16S rRNA\u57fa\u56e0)\u3002\u4f7f\u7528QIIME1.9.1\u5bf9\u9ad8\u901a\u91cf\u771f\u83cc\u6570\u636e\u8fdb\u884c\u5904\u7406\u548c\u5206\u6790\uff0c\u5de5\u4f5c\u6d41\u7a0b\u89c1http:\/\/nbviewer.ipython.org\/github\/biocore\/qiime\/blob\/1.9.1\/examples\/ipynb\/illumina_overview_tutorial.ipynb\u3002\u4f7f\u7528\u5728Greengenes 13_8 16S rRNA\u6570\u636e\u5e93\u4e0a\u8bad\u7ec3\u7684Ribosomal Database Project classifier\uff0c\u9274\u5b9a\u6bcf\u79cd\u7ec6\u83cc\u7684\u7cfb\u7edf\u578b\u3002\u4f7f\u7528UNITE\/QIIME ITS \u53c2\u8003OTU\u6570\u636e\u5e93\u5bf9\u6bcf\u4e2a\u771f\u83ccOTU\u8fdb\u884c\u5206\u7c7b\u9274\u5b9a\u3002\u968f\u673a\u9009\u62e920005\u4e2a\u7ec6\u83cc\u5e8f\u5217\u548c11076\u4e2a\u771f\u83cc\u5e8f\u5217\u8fdb\u884c\u62bd\u5e73\uff0c\u4ee5\u786e\u4fdd\u6240\u6709\u6570\u636e\u96c6\u5747\u5300\u91c7\u6837\u3002<\/p>\n

\u5171\u53d1\u751f\u7f51\u7edc\u5206\u6790<\/h4>\n

\u4f7f\u7528R\u7684WGCNA\u8f6f\u4ef6\u5305\u57fa\u4e8eSPARCC\u76f8\u5173\u77e9\u9635\u8fdb\u884c\u5171\u53d1\u751f\u7f51\u7edc\u5206\u6790\u3002\u4e3a\u4e86\u63d0\u9ad8\u7f51\u7edc\u7684\u53ef\u9760\u6027\uff0c\u5bf9OTU\u8868\u8fdb\u884c\u7b5b\u9009\uff1a\u5728\u6240\u6709\u6837\u672c\u4e2d\u53ea\u4fdd\u7559\u4e86>30%\u7684OTU\uff0c\u6837\u672c\u4e2d\u53ea\u4fdd\u7559\u4e86\u5e73\u5747\u76f8\u5bf9\u4e30\u5ea6>0.01%\u7684OTU\u3002\u5171\u4fdd\u7559\u4e864473\u4e2a\u7ec6\u83ccOTU\u548c685\u4e2a\u771f\u83ccOTU\uff0c\u7528\u6765\u6784\u5efa\u5171\u53d1\u751f\u7f51\u7edc\u3002\u4fee\u6b63\u9519\u8bef\u53d1\u73b0\u7387\u4ee5\u8c03\u6574\u76f8\u5173\u7f51\u7edc\u7684P\u503c\u3002\u5728\u6784\u5efa\u7f51\u7edc\u4e4b\u524d\uff0c\u6d4b\u8bd5\u8054\u5408\u7684\u53ef\u9760\u6027\u3002\u4f7f\u7528R\u7684igraph\u5305\u8ba1\u7b97\u7f51\u7edc\u5c5e\u6027\u3002\u4f7f\u7528Gephi(http:\/\/gephi.github.io\/)\u751f\u6210\u7f51\u7edc\u56fe\u50cf\u3002\u751f\u6210\u8282\u70b9\u548c\u8fb9\u6570\u4e0e\u7ecf\u9a8c\u7f51\u7edc\u76f8\u540c\u7684\u968f\u673a\u7f51\u7edc\uff0c\u5e76\u57fa\u4e8eErd\u00f6s-R\u00e9yni\u6a21\u578b\u901a\u8fc7999\u6b21\u8fed\u4ee3\u603b\u7ed3\u62d3\u6251\u6307\u6570\uff0c\u4ee5\u786e\u5b9a\u7f51\u7edc\u5c5e\u6027\u662f\u5426\u5bb9\u6613\u53d1\u751f\u9519\u8bef\u3002\u6574\u4e2a\u7f51\u7edc\u5305\u542b1239\u4e2a\u8282\u70b9\u548c37203\u6761\u8fb9\u3002\u6211\u4eec\u7684\u76f8\u5173\u7f51\u7edc\u5305\u542b8\u4e2a\u6a21\u5757(**\u56feS2**)\u3002\u7ed3\u6784\u5316\u4e3a\u5fae\u751f\u7269\u6a21\u5757\u7684\u5fae\u751f\u7269\u7fa4\u843d\u8868\u660e\u5177\u6709\u4e0d\u540c\u5173\u8054\u7684\u4e0d\u540c\u751f\u7269\u7fa4\u3002<\/p>\n

\"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790<\/p>\n

\n

\u56feS2 \u534e\u5317\u5e73\u539f\u9ea6\u7530\u571f\u58e4\u5fae\u751f\u7269\u7f51\u7edc<\/p>\n<\/blockquote>\n

\u5728\u672c\u7814\u7a76\u4e2d\uff0c\u6309\u7167Guimera\u548cAmaral(2005)\u63cf\u8ff0\u7684\u65b9\u6cd5\u8ba1\u7b97z-score \u548c c-score\u3002\u4e3a\u4e86\u5f62\u8c61\u5730\u63cf\u8ff0\u8282\u70b9\u7684\u4f5c\u7528\uff0c\u6839\u636e\u5176\u6a21\u5757\u5185\u5ea6(z-score)\u548c\u53c2\u4e0e\u7cfb\u6570(c-score)\u9608\u503c\uff0c\u5212\u5206kinless hubs (z-score > 2.5; c-score > 0.6) \uff0cprovincial hubs (z-score > 2.5; c-score< 0.6), connectors (z-score < 2.5; c-score> 0.6) \u4e0eperipherals (z-score < 2.5; c-score < 0.6)\u3002kinless hubs\u610f\u5473\u7740\u8282\u70b9\u5728\u6a21\u5757\u5185\u548c\u6a21\u5757\u95f4\u90fd\u9ad8\u5ea6\u8fde\u63a5\uff0c\u8fd9\u4e9b\u7c7b\u7fa4\u9884\u8ba1\u4f1a\u4ecb\u5bfc\u8282\u6a21\u5757\u5185\u548c\u6a21\u5757\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u7269\u79cd\uff0c\u5e76\u53ef\u80fd\u901a\u8fc7\u6a21\u5757\u5185\u548c\u6a21\u5757\u4e4b\u95f4\u7684\u80fd\u91cf\u548c\u7269\u8d28\u4ea4\u6362\u800c\u5bf9\u529f\u80fd\u53d1\u6325\u81f3\u5173\u91cd\u8981\uff1bprovincial hubs\u610f\u5473\u7740\u8282\u70b9\u5728\u6a21\u5757\u5185\u9ad8\u5ea6\u8fde\u63a5\uff0c\u8fd9\u4e9b\u7c7b\u7fa4\u6709\u671b\u4ecb\u5bfc\u6a21\u5757\u5185\u7684\u7269\u79cd\u76f8\u4e92\u4f5c\u7528\uff0c\u5e76\u4e14\u53ef\u80fd\u901a\u8fc7\u6a21\u5757\u5185\u7684\u80fd\u91cf\u548c\u7269\u8d28\u7684\u4ea4\u6362\u800c\u5bf9\u529f\u80fd\u884c\u9a76\u975e\u5e38\u91cd\u8981\uff1bConnectors\u5728\u6a21\u5757\u4e4b\u95f4\u9ad8\u5ea6\u8fde\u63a5\uff0c\u8fd9\u4e9b\u7c7b\u7fa4\u6709\u671b\u4ecb\u5bfc\u6a21\u5757\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u7269\u79cd\uff0c\u5e76\u4e14\u5bf9\u4e8e\u5141\u8bb8\u6a21\u5757\u4e4b\u95f4\u8fdb\u884c\u80fd\u91cf\u548c\u7269\u8d28\u4ea4\u6362\u7684\u529f\u80fd\u53ef\u80fd\u975e\u5e38\u91cd\u8981\uff1bperipherals\u610f\u5473\u7740\u8be5\u8282\u70b9\u4e0e\u5176\u4ed6\u8282\u70b9\u7684\u94fe\u63a5\u5f88\u5c11\u6216\u6ca1\u6709\u94fe\u63a5\u3002<\/p>\n

\u7edf\u8ba1\u5206\u6790<\/h4>\n

\u901a\u8fc7\u51cf\u5c11\u4e3b\u8f74\u56de\u5f52\u5206\u6790\u7f51\u7edc\u67a2\u7ebd\u3001\u571f\u58e4\u6027\u8d28\u548c\u529f\u80fd\u57fa\u56e0\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u5e76\u5229\u7528\u57fa\u4e8eAMOS\u7684\u7ed3\u6784\u65b9\u7a0b\u6a21\u578b(SEM)\u63a8\u65ad\u571f\u58e4\u53d8\u91cf\u3001kinless hubs, connectors, provincial hubs \u4e0e peripherals\u5bf9\u571f\u58e4\u529f\u80fd\u7684\u76f4\u63a5\u548c\u95f4\u63a5\u5f71\u54cd\u3002\u6839\u636e\u6240\u6709\u529f\u80fd\u57fa\u56e0\u7684\u5e73\u5747\u6807\u51c6\u5316\u4e30\u5ea6\u8ba1\u7b97\u51fa\u7ed3\u6784\u65b9\u7a0b\u7684\u529f\u80fd\u6f5c\u529b\u3002\u4f7f\u7528kinless- hubs OTUs\u7684\u5e73\u5747\u6807\u51c6\u5316\u4e30\u5ea6\u9274\u522bkinless hubs\u3002\u540c\u7406\uff0c\u5206\u6790connectors, provincial hubs \u4e0e peripherals\u3002<\/p>\n

\u6570\u636e\u548c\u6750\u6599\u7684\u53ef\u7528\u6027<\/h4>\n

\u6570\u636e\u96c6\u4fdd\u5b58\u5728NCBI Sequence Read Archive\uff0c\u7f16\u53f7\u4e3aSRP(\u7ec6\u83cc)\u548cSRP(\u771f\u83cc)\u3002\u66f4\u591a\u6570\u636e\u53ef\u4ee5\u5728\u8865\u5145\u4fe1\u606f\u4e2d\u83b7\u5f97\uff0c\u4e5f\u53ef\u4ee5\u901a\u8fc7\u5408\u7406\u7684\u8bf7\u6c42\u4ece\u76f8\u5e94\u7684\u4f5c\u8005\u90a3\u91cc\u83b7\u5f97\u3002<\/p>\n

\u901a\u8baf\u4f5c\u8005\u7b80\u4ecb<\/h3>\n

\"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790<\/p>\n

\u891a\u6d77\u71d5\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u5357\u4eac\u571f\u58e4\u7814\u7a76\u6240\u7814\u7a76\u5458\uff0c\u535a\u58eb\u751f\u5bfc\u5e08\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u5927\u5b66\u571f\u58e4\u751f\u7269\u5b66\u9996\u5e2d\u6559\u6388<\/strong>\u30022000\u5e74\u4e8e\u4e2d\u56fd\u79d1\u5b66\u9662\u5357\u4eac\u571f\u58e4\u7814\u7a76\u6240\u83b7\u5f97\u535a\u58eb\u5b66\u4f4d\u5e76\u7559\u6240\u5de5\u4f5c\u30022001.06-2003.09\u5728\u65e5\u672c\u56fd\u9645\u519c\u6797\u6c34\u4ea7\u4e1a\u7814\u7a76\u4e2d\u5fc3(JIRCAS)\u4efb\u957f\u671f\u62db\u8058\u7814\u7a76\u5458\u30022004.10-2007.02\u5728\u65e5\u672c\u56fd\u7acb\u519c\u4e1a\u73af\u5883\u6280\u672f\u7814\u7a76\u6240(NIAES)\u4efb\u65e5\u672c\u5b66\u672f\u632f\u5174\u4f1a(JSPS)\u7279\u522b\u7814\u7a76\u5458\u30022007.04-2010.08\u5728\u52a0\u62ff\u5927Queen\u2019s University\u4efb\u7814\u7a76\u52a9\u7406\u30022010\u5e748\u6708\u5168\u804c\u56de\u5230\u5357\u4eac\u571f\u58e4\u7814\u7a76\u6240\u5de5\u4f5c\u3002\u4e3b\u8981\u7814\u7a76\u571f\u58e4\u5fae\u751f\u7269\u751f\u7269\u5730\u7406\u5b66\uff0c\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u4e0e\u529f\u80fd\u5bf9\u5168\u7403\u53d8\u5316\u4e0e\u519c\u4e1a\u7ba1\u7406\u7684\u54cd\u5e94<\/strong>\u3002\u76ee\u524d\u5728ISME J\u3001Microbiome\u7b49\u6742\u5fd7\u53d1\u8868\u8bba\u6587200\u4f59\u7bc7\uff0c\u5176\u4e2dSCI\u8bba\u6587120\u7bc7\uff0cH\u56e0\u5b5038\u3002\u62c5\u4efbISME J\u3001mSystems\u7b496\u79cd\u56fd\u9645\u6742\u5fd7\u7f16\u59d4\uff0c\u4f5c\u4e3a\u5171\u540c\u4e3b\u7f16\u521b\u529e\u4e86Soil Ecology Letters\u6742\u5fd7\u30022019\u5e74\u5165\u9009ESI\u201c\u5168\u7403\u9ad8\u88ab\u5f15\u5b66\u8005\u201d\u3001\u5168\u7403\u201c\u73af\u5883\u79d1\u5b66\u201d\u9ad8\u4ea7\u4f5c\u8005TOP100<\/strong>\u3002<\/p>\n

Reference<\/h3>\n

Yu Shi,Manuel Delgado-Baquerizo,Yuntao Li,Yunfeng Yang,Yong-Guan Zhu,Josep Pe\u00f1uelas,Haiyan Chu. Abundance of kinless hubs within soil microbial networks are associated with high functional potential in agricultural ecosystems.Environment International 142 (2020) https:\/\/doi.org\/10.1016\/j.envint.2020.<\/p>\n

\u4f5c\u8005\u76f8\u5173\u6587\u7ae0<\/h3>\n
    \n
  • \n

    Microbiome\uff1a\u7a7a\u95f4\u5c3a\u5ea6\u5bf9\u534e\u5317\u5e73\u539f\u9ea6\u7530\u571f\u58e4\u7ec6\u83cc\u7fa4\u843d\u7684\u5f71\u54cd(\u5357\u571f\u6240\u891a\u6d77\u71d5\u7ec4)<\/p>\n<\/li>\n

  • \n

    Microbiome\uff1a40\u5e74\u65bd\u80a5\u5904\u7406\u540e\u56fa\u6c2e\u83cc\u53ca\u6c2e\u56fa\u5b9a\u53d7\u6291\u5236<\/p>\n<\/li>\n

  • \n

    mSystems\uff1a\u5357\u4eac\u571f\u58e4\u6240\u891a\u6d77\u71d5\u7ec4\u53d7\u9080\u53d1\u8868\u5fae\u751f\u7269\u751f\u7269\u5730\u7406\u5b66\u7efc\u8ff0(\u5b98\u65b9\u914d\u89c6\u9891\u7b80\u4ecb)<\/p>\n<\/li>\n

  • \n

    mSystems\uff1a\u5357\u571f\u6240\u891a\u6d77\u71d5\u7ec4\u63ed\u793a\u4e86\u5927\u7a7a\u95f4\u5c3a\u5ea6\u4e0b\u7a7a\u95f4\u6bd4\u5b63\u8282\u5bf9\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7684\u5f71\u54cd\u5927<\/p>\n<\/li>\n

  • \n

    SBB\uff1a\u5357\u571f\u6240\u891a\u6d77\u71d5\u7ec4-pH\u4e3b\u5bfc\u571f\u58e4\u4e2d\u56fa\u6c2e\u7fa4\u843d\u7684\u5171\u5b58\u4e0e\u88c5\u914d<\/p>\n<\/li>\n<\/ul>\n

    \u731c\u4f60\u559c\u6b22<\/h3>\n

    10000+\uff1a\u83cc\u7fa4\u5206\u6790 \u5b9d\u5b9d\u4e0e\u732b\u72d7 \u6885\u6bd2\u72c2\u60f3\u66f2 \u63d0DNA\u53d1Nature Cell\u4e13\u520a \u80a0\u9053\u6307\u6325\u5927\u8111<\/p>\n

    \u7cfb\u5217\u6559\u7a0b\uff1a\u5fae\u751f\u7269\u7ec4\u5165\u95e8 Biostar \u5fae\u751f\u7269\u7ec4  \u5b8f\u57fa\u56e0\u7ec4<\/p>\n

    \u4e13\u4e1a\u6280\u80fd\uff1a\u5b66\u672f\u56fe\u8868 \u9ad8\u5206\u6587\u7ae0 \u751f\u4fe1\u5b9d\u5178 \u4e0d\u53ef\u6216\u7f3a\u7684\u4eba<\/p>\n

    \u4e00\u6587\u8bfb\u61c2\uff1a\u5b8f\u57fa\u56e0\u7ec4 \u5bc4\u751f\u866b\u76ca\u5904 \u8fdb\u5316\u6811<\/p>\n

    \u5fc5\u5907\u6280\u80fd\uff1a\u63d0\u95ee \u641c\u7d22  Endnote<\/p>\n

    \u6587\u732e\u9605\u8bfb \u70ed\u5fc3\u80a0 SemanticScholar Geenmedical<\/p>\n

    \u6269\u589e\u5b50\u5206\u6790\uff1a\u56fe\u8868\u89e3\u8bfb \u5206\u6790\u6d41\u7a0b \u7edf\u8ba1\u7ed8\u56fe<\/p>\n

    16S\u529f\u80fd\u9884\u6d4b   PICRUSt  FAPROTAX  Bugbase Tax4Fun<\/p>\n

    \u5728\u7ebf\u5de5\u5177\uff1a16S\u9884\u6d4b\u57f9\u517b\u57fa \u751f\u4fe1\u7ed8\u56fe<\/p>\n

    \u79d1\u7814\u7ecf\u9a8c\uff1a\u4e91\u7b14\u8bb0  \u4e91\u534f\u4f5c \u516c\u4f17\u53f7<\/p>\n

    \u7f16\u7a0b\u6a21\u677f: Shell  R Perl<\/p>\n

    \u751f\u7269\u79d1\u666e:  \u80a0\u9053\u7ec6\u83cc \u4eba\u4f53\u4e0a\u7684\u751f\u547d \u751f\u547d\u5927\u8dc3\u8fdb  \u7ec6\u80de\u6697\u6218 \u4eba\u4f53\u5965\u79d8  <\/p>\n

    \u5199\u5728\u540e\u9762<\/h3>\n

    \u4e3a\u9f13\u52b1\u8bfb\u8005\u4ea4\u6d41\u3001\u5feb\u901f\u89e3\u51b3\u79d1\u7814\u56f0\u96be\uff0c\u6211\u4eec\u5efa\u7acb\u4e86\u201c\u5b8f\u57fa\u56e0\u7ec4\u201d\u4e13\u4e1a\u8ba8\u8bba\u7fa4\uff0c\u76ee\u524d\u5df1\u6709\u56fd\u5185\u59165000+ \u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u52a0\u5165\u3002\u53c2\u4e0e\u8ba8\u8bba\uff0c\u83b7\u5f97\u4e13\u4e1a\u89e3\u7b54\uff0c\u6b22\u8fce\u5206\u4eab\u6b64\u6587\u81f3\u670b\u53cb\u5708\uff0c\u5e76\u626b\u7801\u52a0\u4e3b\u7f16\u597d\u53cb\u5e26\u4f60\u5165\u7fa4\uff0c\u52a1\u5fc5\u5907\u6ce8\u201c\u59d3\u540d-\u5355\u4f4d-\u7814\u7a76\u65b9\u5411-\u804c\u79f0\/\u5e74\u7ea7\u201d\u3002PI\u8bf7\u660e\u793a\u8eab\u4efd\uff0c\u53e6\u6709\u6d77\u5185\u5916\u5fae\u751f\u7269\u76f8\u5173PI\u7fa4\u4f9b\u5927\u4f6c\u5408\u4f5c\u4ea4\u6d41\u3002\u6280\u672f\u95ee\u9898\u5bfb\u6c42\u5e2e\u52a9\uff0c\u9996\u5148\u9605\u8bfb\u300a\u5982\u4f55\u4f18\u96c5\u7684\u63d0\u95ee\u300b\u5b66\u4e60\u89e3\u51b3\u95ee\u9898\u601d\u8def\uff0c\u4ecd\u672a\u89e3\u51b3\u7fa4\u5185\u8ba8\u8bba\uff0c\u95ee\u9898\u4e0d\u79c1\u804a\uff0c\u5e2e\u52a9\u540c\u884c\u3002<\/p>\n

    \"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790<\/p>\n

    \u5b66\u4e6016S\u6269\u589e\u5b50\u3001\u5b8f\u57fa\u56e0\u7ec4\u79d1\u7814\u601d\u8def\u548c\u5206\u6790\u5b9e\u6218\uff0c\u5173\u6ce8\u201c\u5b8f\u57fa\u56e0\u7ec4\u201d<\/p>\n

    \"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790\u70b9\u51fb\u9605\u8bfb\u539f\u6587\uff0c\u8df3\u8f6c\u6700\u65b0\u6587\u7ae0\u76ee\u5f55\u9605\u8bfb<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790_\u571f\u58e4\u5fae\u751f\u7269\u7fa4\u843d\u7ed3\u6784\u5206\u6790\u519c\u4e1a\u571f\u58e4\u5fae\u751f\u7269\u6838\u5fc3\u83cc\u7fa4\u80fd\u591f\u63d0\u5347\u571f\u58e4\u751f\u6001\u529f\u80fdAbundanceofkinlesshubswithinso...","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[],"tags":[],"_links":{"self":[{"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/posts\/8108"}],"collection":[{"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/comments?post=8108"}],"version-history":[{"count":0,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/posts\/8108\/revisions"}],"wp:attachment":[{"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/media?parent=8108"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/categories?post=8108"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/tags?post=8108"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}