{"id":9016,"date":"2024-05-04T17:52:22","date_gmt":"2024-05-04T09:52:22","guid":{"rendered":""},"modified":"2024-05-04T17:52:22","modified_gmt":"2024-05-04T09:52:22","slug":"\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7b56\u7565","status":"publish","type":"post","link":"https:\/\/mushiming.com\/9016.html","title":{"rendered":"\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7b56\u7565"},"content":{"rendered":"<p><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" style=\"display: none;\"> \n <path stroke-linecap=\"round\" d=\"M5,0 0,2.5 5,5z\" id=\"raphael-marker-block\" style=\"-webkit-tap-highlight-color: rgba(0, 0, 0, 0);\"><\/path> \n<\/svg> <\/p>\n<p>\u53c2\u8003\uff1a<br \/> http:\/\/www.pibb.ac.cn\/html\/2015\/2\/20140059.htm<\/p>\n<h2>\u5b9a\u4e49<\/h2>\n<p>\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7684\u672c\u8d28\u662f\u6784\u5efa\u5206\u5b50\u591a\u6837\u6027\u6587\u5e93 \u4ee5\u53ca\u4ece\u6587\u5e93\u4e2d\u7b5b\u9009\u5230\u6027\u72b6\u6709\u6539\u8fdb\u7684\u7a81\u53d8\u4f53\uff0c\u6839\u636e \u6587\u5e93\u6784\u5efa\u539f\u7406\u7684\u4e0d\u540c\uff0c\u53ef\u5206\u4e3a\u968f\u673a\u8fdb\u5316\u3001\u534a\u7406\u6027\u8fdb \u5316\u548c\u7406\u6027\u8fdb\u5316\u4e09\u79cd\u7b56\u7565\uff0c\u5176\u5927\u81f4\u601d\u8def\u5747\u4e3a\u7531\u67d0\u4e00\u9776\u57fa\u56e0\u6216\u4e00\u65cf\u76f8\u5173\u7684\u5bb6\u65cf\u57fa\u56e0\u8d77\u59cb\uff0c\u901a\u8fc7\u5bf9\u7f16\u7801\u57fa\u56e0 \u8fdb\u884c\u7a81\u53d8\u6216\u91cd\u7ec4\uff0c\u521b\u5efa\u5206\u5b50\u591a\u6837\u6027\u6587\u5e93\uff1b\u7b5b\u9009\u6587\u5e93 \u83b7\u5f97\u80fd\u591f\u7f16\u7801\u6539\u8fdb\u6027\u72b6\u7684\u57fa\u56e0\uff0c\u4f5c\u4e3a\u4e0b\u4e00\u8f6e\u8fdb\u5316\u7684 \u6a21\u677f\uff1b\u5728\u77ed\u65f6\u95f4\u5185\u5b8c\u6210\u81ea\u7136\u754c\u4e2d\u9700\u8981\u6210\u5343\u4e0a\u4e07\u5e74\u7684\u8fdb\u5316\uff0c\u4ece\u800c\u83b7\u5f97\u5177\u6709\u6539\u8fdb\u529f\u80fd\u6216\u5168\u65b0\u529f\u80fd\u7684\u86cb\u767d\u8d28\u3002<\/p>\n<h2>\u968f\u673a\u8fdb\u5316<\/h2>\n<p><img decoding=\"async\" src=\"https:\/\/img.mushiming.top\/app\/mushiming_com\/ebea5f62360841b4b7e8d34fdfed7d9a.png\" =\"\" =\"\u86cb\u767d\u8d28\u4f53\u5916\u968f\u673a\u8fdb\u5316\u7684\u4e00\u822c\u6d41\u7a0b\" alt=\"\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7b56\u7565 (https:\/\/mushiming.com\/)  \u7b2c1\u5f20\" title=\"\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7b56\u7565  \u7b2c1\u5f20-\u7a46\u4e16\u660e\u535a\u5ba2\" ><\/p>\n<h4>\u968f\u673a\u8fdb\u5316\u65b9\u6cd5<\/h4>\n<ol>\n<li>\u4f53\u5916\u968f\u673a\u8fdb\u5316<\/li>\n<\/ol>\n<ul>\n<li>\u975e\u91cd\u7ec4\u7684\u4f53\u5916\u968f\u673a\u8fdb\u5316<\/li>\n<li>\u57fa\u4e8e\u91cd\u7ec4\u7684\u4f53\u5916\u968f\u673a\u8fdb\u5316<\/li>\n<\/ul>\n<ol start=\"2\">\n<li>\u57fa\u4e8e\u91cd\u7ec4\u7684\u4f53\u5185\u968f\u673a\u8fdb\u5316<\/li>\n<\/ol>\n<h4>\u57fa\u4e8e\u91cd\u7ec4\u7684\u4f53\u5916\u968f\u673a\u8fdb\u5316<\/h4>\n<h5>\u4e0d\u540c\u968f\u673a\u8fdb\u5316\u7684\u57fa\u672c\u6280\u672f\u6d41\u7a0b<\/h5>\n<p><img decoding=\"async\" src=\"https:\/\/img.mushiming.top\/app\/mushiming_com\/576636f31702420baeb2f7e1df12ee2c.png\" =\"\" =\"\u5728\u8fd9\u91cc\u63d2\u5165\u56fe\u7247\u63cf\u8ff0\" alt=\"\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7b56\u7565 (https:\/\/mushiming.com\/)  \u7b2c2\u5f20\" title=\"\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7b56\u7565  \u7b2c2\u5f20-\u7a46\u4e16\u660e\u535a\u5ba2\" ><br \/> (a) \u6613\u9519PCR\uff0c\u6539\u53d8PCR\u53cd\u5e94\u6761\u4ef6\uff0c\u4ea7\u751f\u968f\u673a\u7a81\u53d8.<br \/> (b) \u70b9\u9971\u548c\u7a81\u53d8\uff0c\u5c06\u6a21\u677fDNA\u7684\u7279\u5b9a\u4f4d\u70b9\u7a81\u53d8\u4e3a\u6240\u670920 \u79cd\u6c28\u57fa\u9178.<br \/> \u00a9 DNA\u91cd\u7ec4\uff0c\u5c06\u4e00 \u7ec4\u540c\u6e90\u57fa\u56e0\u7528\u6838\u9178\u9176\u6d88\u5316\uff0c\u5f97\u5230\u7684\u968f\u673a\u4ea7\u7269\u4e92\u4e3a\u5f15\u7269\u548c\u6a21\u677f\u8fdb\u884cPCR \u6269\u589e\uff0c\u5f53\u6765\u6e90\u4e0d\u540c\u7684\u7247\u6bb5\u4e4b\u95f4\u76f8\u4e92\u5f62\u6210\u6a21\u677f\u65f6\uff0c\u5373\u53d1\u751f\u91cd\u7ec4.<br \/> (d) \u4e32\u8054\u91cd\u590d\u63d2\u5165\uff0c\u5c06\u4e00\u7ec4\u57fa\u56e0\u7528CircLigase \u6d88\u5316\uff0c\u518d\u4f7f\u7528\u8fde\u63a5\u9176\u5c06\u5176\u8fde\u63a5\u6210\u73af\uff0c\u5e76\u4ee5\u6b64\u4f5c\u4e3a\u6a21\u677f\u8fdb\u884cPCR\u53cd\u5e94\uff0c\u4e0d\u540c\u7684\u4e32\u8054\u91cd\u590d\u5e8f\u5217\u53d1\u751f\u968f\u673a \u8fde\u63a5\uff0c\u5f97\u5230\u76ee\u7684\u6587\u5e93.<\/p>\n<h4>\u57fa\u4e8e\u91cd\u7ec4\u7684\u4f53\u5185\u968f\u673a\u8fdb\u5316<\/h4>\n<h5>\u4f5c\u7528<\/h5>\n<p>\u4f53\u5185\u91cd\u7ec4\u65b9\u6cd5\u5219\u80fd\u591f\u5e73\u884c\u64cd\u4f5c\u591a\u4e2a\u57fa\u56e0\uff0c\u540c\u65f6\u8fdb\u5316\u4ee3\u8c22\u7f51\u7edc\u4e2d\u7684\u591a\u4e2a\u5173\u952e\u86cb\u767d\u9176\uff0c\u4ece\u800c\u5728\u77ed\u65f6\u95f4\u5185\u83b7\u5f97\u8868\u578b\u4f18\u826f\u7684\u7a81\u53d8\u682a\u3002\uff08\u80fd\u5bf9\u4ee3\u8c22\u9014\u5f84\u6216\u7f51\u7edc\u8fdb\u884c\u64cd\u4f5c\uff09<\/p>\n<h5>\u591a\u5143\u57fa\u56e0\u7ec4\u5de5\u7a0b (multiplex automated genome engineering,MAGE)<\/h5>\n<p>\u80fd\u591f\u5bf9\u591a\u4e2a\u57fa\u56e0\u8fdb\u884c\u4fee\u9970\uff0c\u8fbe\u5230\u8fdb\u5316\u4ee3\u8c22\u9014\u5f84\u751a\u81f3 \u6574\u4e2a\u7ec6\u80de\u6027\u80fd\u7684\u76ee\u7684\uff0eMAGE \u57fa\u4e8essDNA \u540c\u6e90\u91cd\u7ec4\u7684\u539f\u7406\uff0c\u5c06\u5408\u6210\u7684ssDNA \u5bfc\u5165\u7ec6\u80de\u5e76\u5b9a\u4f4d\u5728\u67d3\u8272\u4f53\u7684\u7279\u5b9a\u4f4d\u70b9\uff0c\u4f7f\u5f97\u5230\u7684\u6bcf\u4e2a\u7ec6\u80de\u90fd\u542b\u6709\u4e00\u7ec4\u4e0d\u540c\u7684\u7a81\u53d8\uff0c\u91cd\u590d\u8fdb\u884c\u6b64\u8fc7\u7a0b\u53ef\u4ee5\u5feb\u901f\u4e14\u8fde\u7eed\u5730\u83b7\u5f97\u4e30\u5bcc\u7684\u5e8f\u5217\u591a\u6837\u6027\u5f02\u8d28\u7fa4\uff0c\u6700\u540e\u9274\u5b9a\u7ec6\u80de\u57fa\u56e0\u578b\u548c\u8868\u578b\uff0e\u4f18\u5316\u6761\u4ef6\u540e\uff0c\u4f7f\u7528MAGE \u6280\u672f\u53ef\u57282\uff5e2.5 h \u5185\u5bf9\u76ee\u6807\u7ec6\u80de\u7fa4\u4f53\u5f15\u5165\u81f3\u5c1130%\u7684\u57fa\u56e0\u4fee\u9970\uff0e<\/p>\n<ul>\n<li>\u5e94\u7528\u5b9e\u4f8b<br \/> Harris\u7b49\u7528\u957f\u5ea6\u4e3a90-mer \u7684\u6838\u7cd6\u4f53\u7ed3\u5408\u4f4d\u70b9(RBS) \u6587\u5e93(DDRRRRRDDDD\uff1bD=A\uff0cG\uff0cT\uff1bR=A\uff0cG) \u5bf91- \u8131\u6c27-D- \u6728\u916e\u7cd6-5- \u78f7\u9178\u8fd8\u539f\u5f02\u6784\u9176\u5408\u6210\u9014\u5f84 (DXP \u5408\u6210\u9014\u5f84)\u4e2d20 \u79cd\u57fa\u56e0\u7684RBS \u533a\u57df\u8fdb\u884c\u7f6e \u6362\uff0c\u4f18\u5316\u4e0d\u540c\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\uff0e\u540c\u65f6\uff0c\u4e3a\u63d0\u9ad8 DXP \u5408\u6210\u9014\u5f84\u901a\u91cf\uff0c\u5c06\u5176\u6ea2\u6d41\u4ee3\u8c22\u9014\u5f84\u4e2d4 \u4e2a\u57fa \u56e0(ytjC\u3001fdhF\u3001aceE\u3001gdhA)\u7684\u5f00\u653e\u9605\u8bfb\u6846(ORF)\u5206 \u522b\u5f15\u5165\u4e86\u4e24\u4e2a\u65e0\u4e49\u7a81\u53d8\uff0e\u7ecf\u8fc735 \u8f6eMAGE \u5faa\u73af\uff0c \u4ece\u591a\u8fbe150\u4ebf\u79cd\u7a81\u53d8\u4f53\u6587\u5e93\u4e2d\u7b5b\u9009\u5230\u4e86\u5c06\u756a\u8304\u7ea2\u7d20 \u7684\u4ea7\u80fd\u63d0\u9ad85 \u500d\u7684\u7a81\u53d8\u682a\uff0e<\/li>\n<\/ul>\n<h5>\u591a\u5143\u8d28\u7c92\u5de5\u7a0b(multiplex iterative plasmid engineering\uff0cMIPE)\u6280\u672f<\/h5>\n<p>\u53c2\u8003 MAGE \u6280\u672f\u7684\u539f\u7406\uff0c\u4ee5\u5355\u94fessDNA \u4ecb\u5bfc\u7684\u03bb-red \u91cd\u7ec4\uff0c\u53ef\u4ee5\u540c\u65f6\u4fee\u6539\u8d28\u7c92\u4e0a\u7684\u591a\u4e2a\u4f4d\u70b9\uff0e\u901a\u8fc7\u9176\u5207 \u5171\u7b5b\u9009\u6280\u672f\uff0c\u63d0\u9ad8\u4e86\u591a\u7247\u6bb5\u91cd\u7ec4\u6548\u7387\uff0c6 bp \u7684\u9176\u5207 \u4f4d\u70b9\u53ef\u4ee5\u88ab\u53cd\u590d\u5229\u7528\uff0c\u65e0\u9650\u5faa\u73af\uff0eMIPE \u6280\u672f\u7684\u7a81 \u53d8\u8fc7\u7a0b\u548c\u7b5b\u9009\u8fc7\u7a0b\u4e25\u683c\u5206\u5f00\uff0c\u8fd9\u4f7f\u5b83\u80fd\u591f\u5e94\u7528\u5230\u6ca1 \u6709\u9ad8\u6548\u91cd\u7ec4\u7cfb\u7edf\u7684\u5bbf\u4e3b\u4e2d\uff0e\u6211\u4eec\u5229\u7528MIPE\u6280\u672f\u5bf9 \u7ea2\u8272\u8367\u5149\u86cb\u767d(red fluorescent protein\uff0cRFP)\u4e0a\u76847 \u4e2a\u5173\u952e\u4f4d\u70b9\u8fdb\u884c\u7a81\u53d8\uff0c\u4ec5\u75283 \u5929\u65f6\u95f4\uff0c\u83b7\u5f97\u4e86\u4e0d\u540c \u5149\u8c31\u7279\u6027\u548c\u4e0d\u540c\u5149\u5f3a\u7684RFP \u7a81\u53d8\u4f53\uff0c\u8fd9\u663e\u793a\u4e86 MIPE \u6280\u672f\u5728\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u4e2d\u7684\u5de8\u5927\u5e94\u7528\u6f5c\u529b\uff0e<\/p>\n<h2>\u534a\u7406\u6027\u8fdb\u5316<\/h2>\n<h3>\u6982\u5ff5<\/h3>\n<p>\u534a\u7406\u6027\u8fdb\u5316 (semi-rational evolution)\u7b56\u7565\u5219\u501f\u52a9\u4e86\u751f\u7269\u4fe1\u606f\u5b66\u65b9 \u6cd5\uff0c\u5728\u5206\u6790\u5927\u91cf\u7684\u86cb\u767d\u8d28\u5e8f\u5217\u6bd4\u5bf9\u4fe1\u606f\uff0c\u4e8c\u7ea7\u7ed3\u6784\u6570\u636e\uff0c\u751a\u6216\u662f\u5728\u540c\u6e90\u5efa\u6a21\u5f97\u5230\u76ee\u7684\u86cb\u767d\u4e09\u7ef4\u7a7a\u95f4\u6784\u8c61\u7684\u57fa\u7840\u4e0a\u66f4\u6709\u9488\u5bf9\u6027\u5730\u5bf9\u86cb\u767d\u8d28\u8fdb\u884c\u6539\u9020\uff0c\u4e0d\u4f46\u63d0\u9ad8\u4e86\u9633\u6027\u7a81\u53d8\u7387\uff0c\u800c\u4e14\u5927\u5927\u7f29\u5c0f\u4e86\u7a81\u53d8\u6587\u5e93\u5bb9\u91cf\uff0c\u66f4\u6613\u4e8e\u7b5b\u9009\uff0e<\/p>\n<h3>\u65b9\u6cd5<\/h3>\n<ul>\n<li>\u901a\u8fc7\u8ba1\u7b97\u673a\u6a21\u62df\u83b7\u5f97\u6f5c\u5728\u7684\u6709\u76ca\u7a81\u53d8\u4f4d\u70b9<\/li>\n<li>\u5229\u7528\u9002\u5f53\u7684\u9971\u548c\u7a81\u53d8\u6280\u672f\u6784\u5efa\u5408\u9002\u7684\u7a81\u53d8\u6587\u5e93<\/li>\n<li>\u4e0d\u540c\u7684\u8ba1\u7b97\u673a\u7b97\u6cd5\u4ee5\u53ca\u7ed3\u6784\u5206\u6790\u65b9\u6cd5\uff0e\u6b64\u5916\uff0c\u5bf9\u4e8e\u7ed3\u6784\u8f83\u4e3a\u590d\u6742\u7684\u86cb\u767d\u8d28\uff0c\u53ef\u5c06\u5176\u5206\u4e3a\u4e0d\u540c\u7684\u7ed3\u6784\u5355\u5143\uff0c\u5e76\u5728\u5176\u5185\u90e8\u72ec\u7acb\u8fdb\u5316\uff0c\u7ec4\u5408\u7b5b\u9009\u6700\u4f73\u8fdb\u5316\u5355\u5143\uff0c\u5f97\u5230\u5b8c\u6574\u86cb\u767d\u8d28\uff0e<\/li>\n<\/ul>\n<h4>\u8ba1\u7b97\u673a\u7b97\u6cd5<\/h4>\n<ol>\n<li>\u57fa\u4e8e\u4e09\u7ef4\u7ed3\u6784\u6216\u540c\u6e90\u5efa\u6a21\u5206\u6790<\/li>\n<\/ol>\n<ul>\n<li>\u7a81\u53d8\u4f4d\u70b9\u83b7\u5f97\uff1aSCHEMA\u3001HotSpot Wizard\u3001ProSAR<\/li>\n<li>\u6587\u5e93\u6784\u5efa\u65b9\u6cd5\uff1aGSSM\u3001ISM\u3001CASTing<\/li>\n<li>\u6539\u8fdb\u6027\u8d28\uff1a\u5bf9\u6620\u9009\u62e9\u6027\u3001\u70ed\u7a33\u5b9a\u6027\u3001\u5e95\u7269\u7279\u5f02\u6027<\/li>\n<\/ul>\n<ol start=\"2\">\n<li>\u57fa\u4e8e\u6027\u8d28<br \/> \u7a81\u53d8\u4f4d\u70b9\u83b7\u5f97\uff1aQSAR\u3001ASRA<br \/> 3.\u57fa\u4e8e\u7ed3\u6784\u5206\u6790<\/li>\n<\/ol>\n<ul>\n<li>\u7a81\u53d8\u4f4d\u70b9\u83b7\u5f97\uff1aSCOPE<\/li>\n<li>\u6587\u5e93\u6784\u5efa\u65b9\u6cd5\uff1a\u5916\u663e\u5b50\u91cd\u6392<\/li>\n<li>\u6539\u8fdb\u6027\u8d28\uff1a\u914d\u5408\u57fa-\u53d7\u4f53<\/li>\n<\/ul>\n<h2>\u7406\u6027\u8fdb\u5316<\/h2>\n<h3>\u6982\u5ff5<\/h3>\n<p>\u7406\u6027\u8fdb\u5316\u7b56\u7565\u4e3b\u8981\u662f\u5728\u8ba1\u7b97\u673a\u4e2d(in silico)\u5b8c\u6210\uff0e\u901a\u8fc7\u8ba1\u7b97\u673a\u5efa\u6a21(computational modeling)\u9884 \u6d4b\u86cb\u767d\u8d28\u6d3b\u6027\u4f4d\u70b9\uff0c\u8003\u5bdf\u67d0\u57fa\u56e0\u7a81\u53d8\u5bf9\u76ee\u6807\u86cb\u767d\u7a33\u5b9a\u6027\u3001\u6298\u53e0\u4ee5\u53ca\u4e0e\u5e95\u7269\u7ed3\u5408\u7684\u5f71\u54cd\uff0c\u4ece\u800c\u5bf9\u86cb\u767d\u8d28\u8fdb\u5316\u8fdb\u884c\u8bbe\u8ba1\u6307\u5bfc\u548c\u6a21\u62df\u7b5b\u9009\u5b9e\u9a8c\uff0c\u63d0\u9ad8\u5b9e\u9a8c\u7684\u6210\u529f\u7387\uff0e<\/p>\n<h3>\u6b65\u9aa4<\/h3>\n<ul>\n<li>\u4ece\u5934\u8bbe\u8ba1\u5219\u80fd\u591f\u5bf9\u8fd9\u4e9b\u56e0\u7d20\u52a0\u4ee5\u8003\u8651\uff0e\u4ece\u5934\u8bbe\u8ba1\u9996\u5148\u6839\u636e\u91cf\u5b50\u529b\u5b66\u5efa\u6a21\u5f97\u5230\u76ee\u7684\u50ac\u5316\u53cd\u5e94<\/li>\n<li>\u989d\u5916\u8003\u8651\u67d0\u4e2a\u5177\u6709\u9ad8\u80fd\u5792\u7684\u53cd\u5e94\uff0c\u63a8\u6d4b\u5176\u8fc7\u6e21\u6001\uff0c\u5b9a\u4f4d\u6240\u9700\u7684\u50ac\u5316\u4fa7\u94fe\u5e76\u7ed3\u5408\u53cd\u5e94\u7684\u4f18\u5316\u8fc7\u6e21\u6001<\/li>\n<li>\u5229\u7528QM\/MM\u6a21\u578b\u5206\u6790\u5f97\u5230\u5305\u542b\u8fc7\u6e21\u6001\u548c\u6d89\u53ca\u7ed3\u5408\u3001\u50ac\u5316\u7684\u86cb\u767d\u8d28\u529f\u80fd\u56e2\u7684\u7406\u8bba\u86cb\u767d\u8d28\u7a81\u53d8\u6587\u5e93\uff0c\u518d\u5229\u7528Rosetta\u3001ORBIT\u3001PyMol \u7b49\u8f6f\u4ef6\uff0c\u5728\u5927\u91cf\u7a33\u5b9a\u7684\u86cb\u767d\u652f\u67b6\u4e2d\u641c\u7d22\u80fd\u591f\u652f\u6301\u8fd9\u4e9b\u7406 \u60f3\u6d3b\u6027\u4f4d\u70b9\u7684\u86cb\u767d\u8d28\u4e3b\u94fe\u7fa4\uff0c\u6700\u7ec8\u7ecf\u8fc7\u4f18\u5316\u5904\u7406\u7684\u57fa\u56e0\u5e8f\u5217\u5373\u53ef\u8fdb\u884c\u5b9e\u9a8c\u9a8c\u8bc1<\/li>\n<li>\u8fd9\u79cd\u65b9\u6cd5\u80fd\u591f\u5728\u6570 \u767e\u4e2a\u6f5c\u5728\u7684\u6a21\u677f\u9176\u4fa7\u94fe\u4e2d\u81ea\u52a8\u6311\u9009\u5408\u9002\u7684\u7b97\u6cd5\u641c\u7d22\u50ac\u5316\u4fa7\u94fe\u8fc7\u6e21\u6001\uff0c\u5e76\u5c06\u5176\u5b9a\u4f4d\u5230\u5408\u9002\u4f4d\u7f6e<\/li>\n<\/ul>\n<h3>\u610f\u4e49<\/h3>\n<p>\u4ece\u5934\u8bbe\u8ba1\u5bf9\u4e8e\u9176\u8fdb\u5316\u8f68\u8ff9\u7684\u5206\u6790\u6709\u52a9\u4e8e\u589e\u5f3a\u4eba \u4eec\u5bf9\u86cb\u767d\u8d28\u81ea\u7136\u8fdb\u5316\u7684\u7406\u89e3\uff0c\u4ece\u5b9e\u9a8c\u4e2d\u83b7\u5f97\u7684\u53cd\u9988\u4fe1\u606f\u4e5f\u53ef\u4ee5\u66f4\u597d\u5730\u8f85\u52a9\u5b8c\u5584\u7406\u6027\u8bbe\u8ba1\uff0e\u6b64\u5916\uff0c\u5229\u7528\u8ba1\u7b97\u673a\u9884\u6d4b\u53ef\u4ee5\u5efa\u7acb\u4ee3\u8c22\u9014\u5f84\u3001\u5206\u6790\u4ee3\u8c22\u7f51\u7edc\uff0c\u4ece\u800c\u4f7f\u7528\u5de5\u7a0b\u5b66\u65b9\u6cd5\u9009\u51fa\u91cd\u8981\u7684\u9176\u52a0\u4ee5\u8fdb\u5316\uff0c\u4ee5\u8fbe\u5230\u6574\u4e2a\u9014\u5f84\u6216\u7f51\u7edc\u7684\u4f18\u5316\u76ee\u7684\uff0e<\/p>\n<h2>\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u6587\u5e93\u7684\u7b5b\u9009\u7b56\u7565<\/h2>\n<ul>\n<li>\u8868\u578b\u89c2\u5bdf\u7684\u7b5b\u9009<\/li>\n<li>\u566c\u83cc\u4f53\u8868\u9762\u5c55\u793a(phage display)<\/li>\n<li>\u9175\u6bcd\u8868\u9762\u5c55\u793a(yeast surface display)<\/li>\n<li>\u6d41\u5f0f\u7ec6\u80de\u5206\u9009\u6280\u672f(flowcytometer, FCM)<\/li>\n<\/ul>\n<h3>\u8868\u578b\u89c2\u5bdf\u7684\u7b5b\u9009<\/h3>\n<p>\u901a\u8fc7\u5bf9\u7ec6\u80de\u7684\u751f\u957f\u7387\u3001\u751f\u5b58\u7387\u6216\u5e95\u7269\u6d88\u8017\u3001\u4ea7\u7269\u751f\u6210\u901f \u7387\u7b49\u7684\u89c2\u6d4b\u7b5b\u9009\u51fa\u76ee\u7684\u83cc\u682a\uff0c\u4f8b\u5982\u743c\u8102\u5e73\u677f\u514b\u9686\u7b5b \u9009\u6216\u7c97\u9176\u88c2\u89e3\u6db2\u7684\u9176\u6d3b\u68c0\u6d4b\u7b49\u65b9\u6cd5<\/p>\n<h3>\u566c\u83cc\u4f53\u8868\u9762\u5c55\u793a(phage display)<\/h3>\n<h3>\u9175\u6bcd\u8868\u9762\u5c55\u793a(yeast surface display)<\/h3>\n<p>\u5c06\u8868\u8fbe\u7684\u591a\u80bd\u4ee5\u878d\u5408\u86cb\u767d\u5f62\u5f0f \u5c55\u73b0\u5728\u6838\u7cd6\u4f53\u3001\u75c5\u6bd2\u6216\u7ec6\u80de\u8868\u9762\uff0c\u5e76\u4f7f\u5176\u4fdd\u6301\u76f8\u5bf9 \u72ec\u7acb\u7684\u7a7a\u95f4\u7ed3\u6784\u548c\u751f\u7269\u6d3b\u6027\uff0c\u501f\u4ee5\u7814\u7a76\u591a\u80bd\u7684\u6027 \u8d28\u3001\u76f8\u4e92\u8bc6\u522b\u548c\u4f5c\u7528\uff0c\u7b5b\u9009\u7279\u5b9a\u529f\u80fd\u7684\u591a\u80bd\u7ed3\u6784\uff0c \u5b9e\u73b0\u86cb\u767d\u8d28\u7684\u5b9a\u5411\u8fdb\u5316<\/p>\n<h3>\u6d41\u5f0f\u7ec6\u80de\u5206\u9009\u6280\u672f(flowcytometer, FCM)<\/h3>\n<p>\u6d41\u5f0f\u7ec6\u80de\u5206\u9009\u6280\u672f\u80fd\u591f\u5b9e \u73b0\u5355\u7ec6\u80de\u7684\u4f9d\u6b21\u9ad8\u901f\u901a\u8fc7\u6fc0\u5149\u805a\u7126\u76d1\u6d4b\u70b9\uff0c\u7ecf\u6fc0\u53d1 \u5149\u6fc0\u53d1\u540e\u4ea7\u751f\u8367\u5149\u4fe1\u53f7\uff0c\u6839\u636e\u5149\u4fe1\u53f7\u7684\u53d8\u5316\u6765\u5224\u65ad \u8be5\u7ec6\u80de\u7684\u5927\u5c0f\u3001\u5f62\u6001\u4ee5\u53ca\u8367\u5149\u5f3a\u5ea6\uff0c\u5e76\u53ef\u4ee5\u5c06\u9700\u8981 \u5f97\u5230\u7684\u7ec6\u80de\u4e9a\u7fa4\u4ece\u4e2d\u5206\u9009\u51fa\u6765\uff0e\uff08\u5fae\u6d41\u63a7\u6280\u672f(droplet microfluidic technology)\uff09<\/p>\n<h2>\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7684\u5e94\u7528<\/h2>\n<h3>\u6539\u5584\u9176\u50ac\u5316\u6548\u7387<\/h3>\n<ul>\n<li>\u63d0\u9ad8\u9176\u6d3b\u6027<\/li>\n<li>\u89e3\u9664\u6291\u5236\u4f5c\u7528\uff08\u6b8b\u57fa\u8fdb\u884c\u70b9\u7a81\u53d8\uff09<\/li>\n<\/ul>\n<h3>\u63d0\u9ad8\u9176\u7a33\u5b9a\u6027<\/h3>\n<ul>\n<li>pH \u503c\u7684\u7a33\u5b9a\u6027<\/li>\n<li>\u70ed\u7a33\u5b9a\u6027<\/li>\n<\/ul>\n<h3>\u6539\u53d8\u5e95\u7269\u7279\u5f02\u6027<\/h3>\n<h3>\u521b\u9020\u65b0\u578b\u50ac\u5316\u6d3b\u6027\u86cb\u767d\u9176<\/h3>\n","protected":false},"excerpt":{"rendered":"\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7b56\u7565\u53c2\u8003\uff1ahttp:\/\/www.pibb.ac.cn\/html\/2015\/2\/20140059.htm\u5b9a\u4e49\u86cb\u767d\u8d28\u5b9a\u5411\u8fdb\u5316\u7684\u672c\u8d28\u662f\u6784\u5efa\u5206\u5b50\u591a\u6837\u6027\u6587\u5e93\u4ee5...","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[],"tags":[],"_links":{"self":[{"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/posts\/9016"}],"collection":[{"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/comments?post=9016"}],"version-history":[{"count":0,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/posts\/9016\/revisions"}],"wp:attachment":[{"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/media?parent=9016"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/categories?post=9016"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mushiming.com\/wp-json\/wp\/v2\/tags?post=9016"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}